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Basic Characteristics of Mutations
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Mutation Site
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C483Y |
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Mutation Site Sentence
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Using chikungunya virus (CHIKV), we describe a unique arbovirus fidelity variant with a single C483Y amino acid change in the nsP4 RdRp that increases replication fidelity and generates populations with reduced genetic diversity. |
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Mutation Level
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Amino acid level |
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Mutation Type
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Nonsynonymous substitution |
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Gene/Protein/Region
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nsP4 |
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Standardized Encoding Gene
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nsP4
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Genotype/Subtype
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- |
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Viral Reference
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-
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Functional Impact and Mechanisms
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Disease
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Chikungunya Fever
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Immune
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- |
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Target Gene
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-
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Clinical and Epidemiological Correlations
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Clinical Information
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- |
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Treatment
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- |
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Location
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- |
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Literature Information
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PMID
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21896755
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Title
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Arbovirus high fidelity variant loses fitness in mosquitoes and mice
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Author
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Coffey LL,Beeharry Y,Borderia AV,Blanc H,Vignuzzi M
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Journal
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Proceedings of the National Academy of Sciences of the United States of America
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Journal Info
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2011 Sep 20;108(38):16038-43
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Abstract
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The error rate of RNA-dependent RNA polymerases (RdRp) affects the mutation frequency in a population of viral RNAs. Using chikungunya virus (CHIKV), we describe a unique arbovirus fidelity variant with a single C483Y amino acid change in the nsP4 RdRp that increases replication fidelity and generates populations with reduced genetic diversity. In mosquitoes, high fidelity CHIKV presents lower infection and dissemination titers than wild type. In newborn mice, high fidelity CHIKV produces truncated viremias and lower organ titers. These results indicate that increased replication fidelity and reduced genetic diversity negatively impact arbovirus fitness in invertebrate and vertebrate hosts.
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Sequence Data
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-
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