RABV Mutation Detail Information

Virus Mutation RABV Mutation C54F


Basic Characteristics of Mutations
Mutation Site C54F
Mutation Site Sentence This revealed several interesting features: (i) some mutations were observed both during in vivo and in vitro experiments (Fig 3, red characters: mutations H to Q in position 38 of the P protein (P-H38Q), P-L39R, M-G57R or E, L-L65Q, L-T612I, L-Y613X, L-K1234R); (ii) approximately 50% of these mutations have a frequency among global RABV populations of less than 10% (light yellow rectangles); (iii) in some passages mutations appear in both the brain and the salivary glands and are further transmitted in the following passages, the most significant of which were: M-F150L in 'vDog on dog'; N-N61I and G-C480F in 'vDog on fox'; M-C54F, L-P4S and L-P275L in 'vFox on fox'; (iv) all mutations whose frequency was above 50% were observed both in the brain and in the salivary glands, although this was not always the case for other minor variants; and (v) very few mutations are present in all the replicates (in vivo: N-A97E; in vitro: P-H38Q, P-L39R).
Mutation Level Amino acid level
Mutation Type Nonsynonymous substitution
Gene/Protein/Region M
Standardized Encoding Gene M
Genotype/Subtype Ariana2;91047FRA
Viral Reference KX148127;KF155001.1
Functional Impact and Mechanisms
Disease Rabies Virus infection    
Immune -
Target Gene -
Clinical and Epidemiological Correlations
Clinical Information -
Treatment -
Location Tunisia;France
Literature Information
PMID 31220188
Title Comparison of intra- and inter-host genetic diversity in rabies virus during experimental cross-species transmission
Author Bonnaud EM,Troupin C,Dacheux L,Holmes EC,Monchatre-Leroy E,Tanguy M,Bouchier C,Cliquet F,Barrat J,Bourhy H
Journal PLoS pathogens
Journal Info 2019 Jun 20;15(6):e1007799
Abstract The development of high-throughput genome sequencing enables accurate measurements of levels of sub-consensus intra-host virus genetic diversity and analysis of the role played by natural selection during cross-species transmission. We analysed the natural and experimental evolution of rabies virus (RABV), an important example of a virus that is able to make multiple host jumps. In particular, we (i) analyzed RABV evolution during experimental host switching with the goal of identifying possible genetic markers of host adaptation, (ii) compared the mutational changes observed during passage with those observed in natura, and (iii) determined whether the colonization of new hosts or tissues requires adaptive evolution in the virus. To address these aims, animal infection models (dog and fox) and primary cell culture models (embryo brain cells of dog and fox) were developed and viral variation was studied in detail through deep genome sequencing. Our analysis revealed a strong unidirectional host evolutionary effect, as dog-adapted rabies virus was able to replicate in fox and fox cells relatively easily, while dogs or neuronal dog cells were not easily susceptible to fox adapted-RABV. This suggests that dog RABV may be able to adapt to some hosts more easily than other host variants, or that when RABV switched from dogs to red foxes it lost its ability to adapt easily to other species. Although no difference in patterns of mutation variation between different host organs was observed, mutations were common following both in vitro and in vivo passage. However, only a small number of these mutations also appeared in natura, suggesting that adaptation during successful cross-species virus transmission is a complex, multifactorial evolutionary process.
Sequence Data MK981888;KX148127
Mutation Information
Note
Basic Characteristics of Mutations
  • Mutation Site: The specific location in a gene or protein sequence where a change occurs.
  • Mutation Level: The level at which a mutation occurs, including the nucleotide or amino acid level.
  • Mutation Type: The nature of the mutation, such as missense mutation, nonsense mutation, synonymous mutation, etc.
  • Gene/Protein/Region: Refers to the specific region of the virus where the mutation occurs. Including viral genes, viral proteins, or a specific viral genome region. If the article does not specifically indicate the relationship between the mutation and its correspondence, the main
  • Gene/Protein/Region studied in the article is marked.
  • Genotype/Subtype: Refers to the viral genotype or subtype where the mutation occurs. If the article does not specifically indicate the relationship between the mutation and its correspondence, the main Genotype/Subtype studied in the article is marked.
  • Viral Reference: Refers to the standard virus strain used to compare and analyze viral sequences.
Functional Impact and Mechanisms
  • Disease: An abnormal physiological state with specific symptoms and signs caused by viral infection.
  • Immune: The article focuses on the study of mutations and immune.
  • Target Gene: Host genes that viral mutations may affect.
Clinical and Epidemiological Correlations
  • Clinical Information: The study is a clinical or epidemiological study and provides basic information about the population.
  • Treatment: The study mentioned a certain treatment method, such as drug resistance caused by mutations. If the study does not specifically indicate the relationship between mutations and their correspondence treatment, the main treatment studied in the article is marked.
  • Location: The source of the research data.
Literature Information
  • Sequence Data: The study provides the data accession number.