HPV Mutation Detail Information

Virus Mutation HPV Mutation C7531T


Basic Characteristics of Mutations
Mutation Site C7531T
Mutation Site Sentence Three of those variations, T7365C, C7531T and T7791C are not found in other B-lineage LCRs and may therefore account for the higher transcriptional activity of LCR10, as tested below.
Mutation Level Nucleotide level
Mutation Type Nonsynonymous substitution
Gene/Protein/Region LCR
Standardized Encoding Gene
Genotype/Subtype HPV33
Viral Reference M12732.1
Functional Impact and Mechanisms
Disease -
Immune -
Target Gene -
Clinical and Epidemiological Correlations
Clinical Information -
Treatment -
Location -
Literature Information
PMID 30911096
Title Characterization of an HPV33 natural variant with enhanced transcriptional activity suggests a role for C/EBPbeta in the regulation of the viral early promoter
Author Alvarez J,Gagnon D,Coutlee F,Archambault J
Journal Scientific reports
Journal Info 2019 Mar 25;9(1):5113
Abstract The Long Control Region (LCR) of the human papillomavirus (HPV) genome encompasses the early promoter (EP) that drives expression of the viral oncogenes in infected cells and HPV-associated cancers. Here, we report on a natural variant of HPV33 that displays higher EP activity than the prototype in transfected C33A and HeLa cervical carcinoma cells, and in the osteosarcoma U2OS cell line which supports replication of HPV episomes. This increased promoter activity was ascribed to a single nucleotide variation in the LCR, T7791C, in a putative binding site for the transcription factor C/EBPbeta. T7791C abrogated binding of recombinant C/EBPbeta to this site in vitro and stimulated the EP in vivo, suggesting that it abrogates a negatively-acting regulatory element. A second C/EBPbeta binding site was identified in vitro that activated the EP in vivo and whose function and location in the epithelial-specific enhancer is shown to be conserved in the highly prevalent HPV18. These results suggest that C/EBPbeta is both an activator and a repressor of the HPV33 EP, acting via two distinct binding sites. Prediction of C/EBPbeta sites in the LCR of 186 HPV types suggests that C/EBPbeta regulation of the EP is common among high-risk viruses from the alpha genus.
Sequence Data -
Mutation Information
Note
Basic Characteristics of Mutations
  • Mutation Site: The specific location in a gene or protein sequence where a change occurs.
  • Mutation Level: The level at which a mutation occurs, including the nucleotide or amino acid level.
  • Mutation Type: The nature of the mutation, such as missense mutation, nonsense mutation, synonymous mutation, etc.
  • Gene/Protein/Region: Refers to the specific region of the virus where the mutation occurs. Including viral genes, viral proteins, or a specific viral genome region. If the article does not specifically indicate the relationship between the mutation and its correspondence, the main
  • Gene/Protein/Region studied in the article is marked.
  • Genotype/Subtype: Refers to the viral genotype or subtype where the mutation occurs. If the article does not specifically indicate the relationship between the mutation and its correspondence, the main Genotype/Subtype studied in the article is marked.
  • Viral Reference: Refers to the standard virus strain used to compare and analyze viral sequences.
Functional Impact and Mechanisms
  • Disease: An abnormal physiological state with specific symptoms and signs caused by viral infection.
  • Immune: The article focuses on the study of mutations and immune.
  • Target Gene: Host genes that viral mutations may affect.
Clinical and Epidemiological Correlations
  • Clinical Information: The study is a clinical or epidemiological study and provides basic information about the population.
  • Treatment: The study mentioned a certain treatment method, such as drug resistance caused by mutations. If the study does not specifically indicate the relationship between mutations and their correspondence treatment, the main treatment studied in the article is marked.
  • Location: The source of the research data.
Literature Information
  • Sequence Data: The study provides the data accession number.