SARS-CoV-2 Mutation Detail Information

Virus Mutation SARS-CoV-2 Mutation D108G


Basic Characteristics of Mutations
Mutation Site D108G
Mutation Site Sentence Two mutations were identified including D108G and A249V and shown to increase the molecular flexibility of PLPro protein and alter the protein stability, particularly with D108G mutation.
Mutation Level Amino acid level
Mutation Type Nonsynonymous substitution
Gene/Protein/Region PLpro
Standardized Encoding Gene ORF1a  
Genotype/Subtype -
Viral Reference YP_009724389
Functional Impact and Mechanisms
Disease COVID-19    
Immune -
Target Gene -
Clinical and Epidemiological Correlations
Clinical Information -
Treatment -
Location Saudi
Literature Information
PMID 34548835
Title Analyzing the effect of mutations in SARS-CoV2 papain-like protease from Saudi isolates on protein structure and drug-protein binding: Molecular modelling and dynamics studies
Author Alabbas AB,Alamri MA
Journal Saudi journal of biological sciences
Journal Info 2022 Jan;29(1):526-533
Abstract The continuous and rapid development of the severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) virus remains a health concern especially with the emergence of numerous variants and mutations worldwide. As with other RNA viruses, SARS-CoV-2 has a genetically high mutation rate. These mutations have an impact on the virus characteristics, including transmissibility, antigenicity and development of drug and vaccine resistance. This work was pursued to identify the differences that exist in the papain-like protease (PL(Pro)) from 58 Saudi isolates in comparison to the first reported sequence from Wuhan, China and determine their implications on protein structure and the inhibitor binding. PL(pro) is a key protease enzyme for the host cells invasion and viral proteolytic cleavage, hence, it emerges as a valuable antiviral therapeutic target. Two mutations were identified including D108G and A249V and shown to increase the molecular flexibility of PL(Pro) protein and alter the protein stability, particularly with D108G mutation. The effect of these mutations on the stability and dynamic behavior of PL(Pro) structures as well as their effect on the binding of a known inhibitor; GRL0617 were further investigated by molecular docking and dynamic simulation.
Sequence Data -
Mutation Information
Note
Basic Characteristics of Mutations
  • Mutation Site: The specific location in a gene or protein sequence where a change occurs.
  • Mutation Level: The level at which a mutation occurs, including the nucleotide or amino acid level.
  • Mutation Type: The nature of the mutation, such as missense mutation, nonsense mutation, synonymous mutation, etc.
  • Gene/Protein/Region: Refers to the specific region of the virus where the mutation occurs. Including viral genes, viral proteins, or a specific viral genome region. If the article does not specifically indicate the relationship between the mutation and its correspondence, the main
  • Gene/Protein/Region studied in the article is marked.
  • Genotype/Subtype: Refers to the viral genotype or subtype where the mutation occurs. If the article does not specifically indicate the relationship between the mutation and its correspondence, the main Genotype/Subtype studied in the article is marked.
  • Viral Reference: Refers to the standard virus strain used to compare and analyze viral sequences.
Functional Impact and Mechanisms
  • Disease: An abnormal physiological state with specific symptoms and signs caused by viral infection.
  • Immune: The article focuses on the study of mutations and immune.
  • Target Gene: Host genes that viral mutations may affect.
Clinical and Epidemiological Correlations
  • Clinical Information: The study is a clinical or epidemiological study and provides basic information about the population.
  • Treatment: The study mentioned a certain treatment method, such as drug resistance caused by mutations. If the study does not specifically indicate the relationship between mutations and their correspondence treatment, the main treatment studied in the article is marked.
  • Location: The source of the research data.
Literature Information
  • Sequence Data: The study provides the data accession number.