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Basic Characteristics of Mutations
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Mutation Site
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D112G |
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Mutation Site Sentence
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We have determined the neutral pH crystal structure of one such mutant, HA2 112 Asp----Gly. |
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Mutation Level
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Amino acid level |
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Mutation Type
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Nonsynonymous substitution |
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Gene/Protein/Region
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HA2 |
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Standardized Encoding Gene
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HA
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Genotype/Subtype
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- |
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Viral Reference
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-
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Functional Impact and Mechanisms
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Disease
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-
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Immune
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- |
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Target Gene
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-
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Clinical and Epidemiological Correlations
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Clinical Information
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- |
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Treatment
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- |
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Location
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- |
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Literature Information
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PMID
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2295311
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Title
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The structure of a membrane fusion mutant of the influenza virus haemagglutinin
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Author
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Weis WI,Cusack SC,Brown JH,Daniels RS,Skehel JJ,Wiley DC
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Journal
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The EMBO journal
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Journal Info
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1990 Jan;9(1):17-24
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Abstract
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The haemagglutinin glycoprotein (HA) of influenza virus specifically mediates fusion of the viral and host cell endosomal membranes at the acidic pH of endosomes. The HAs from mutant viruses with raised fusion pH optima contain amino acid substitutions in regions of the HA structure thought to be involved in the fusion process [Daniels et al. (1985b) Cell, 40, 431-439]. We have determined the neutral pH crystal structure of one such mutant, HA2 112 Asp----Gly. A water molecule appears to partially replace the aspartate side chain, and no changes are observed in the surrounding structure. It appears that four intra-chain hydrogen bonds that stabilize the location of the N-terminus of HA2 are lost in the mutant, resulting in a local destabilization that facilitates the extrusion of the N-terminus at higher pH.
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Sequence Data
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-
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