HPV Mutation Detail Information

Virus Mutation HPV Mutation D45E


Basic Characteristics of Mutations
Mutation Site D45E
Mutation Site Sentence In addition, nine amino acid mutations (A7V, A7P, L16I, D45E, L59I, L59T, Q66P, S72F, H75Q) were detected in the E1( )E4 protein, and these were associated with the severity of cervical malignancy.
Mutation Level Amino acid level
Mutation Type Nonsynonymous substitution
Gene/Protein/Region E1^E4
Standardized Encoding Gene E1^E4  
Genotype/Subtype HPV16
Viral Reference NC_ 001526
Functional Impact and Mechanisms
Disease Cervical Intraepithelial Neoplasia    
Immune -
Target Gene -
Clinical and Epidemiological Correlations
Clinical Information -
Treatment -
Location Greece
Literature Information
PMID 22684534
Title Molecular and phylogenetic analysis of the HPV 16 E4 gene in cervical lesions from women in Greece
Author Tsakogiannis D,Ruether IG,Kyriakopoulou Z,Pliaka V,Skordas V,Gartzonika C,Levidiotou-Stefanou S,Markoulatos P
Journal Archives of virology
Journal Info 2012 Sep;157(9):1729-39
Abstract The HPV16 E1(wedge)E4 protein is thought to contribute to the release of newly formed viral particles from infected epithelia. In order to investigate amino acid mutations in the HPV16 E1(wedge)E4 protein, the complete E4 ORF was amplified by PCR in 27 HPV16-positive cervical samples, and the amplicons were cloned. Fifteen nucleic acid variations were identified in the E4 ORF, including seven silent nucleic acid mutations. In addition, nine amino acid mutations (A7V, A7P, L16I, D45E, L59I, L59T, Q66P, S72F, H75Q) were detected in the E1(wedge)E4 protein, and these were associated with the severity of cervical malignancy. A maximum-likelihood phylogenetic tree was constructed based on the E4 ORF, and nucleotide sequence analysis of the E4, E6 and E7 genes from the same samples was conducted in order to determine the phylogenetic origin of the cloned sequences from the amplified HPV16 E4. Based on the nucleotide sequence and phylogenetic analysis it was revealed that even though E4 ORF constitutes a small polymorphic portion of the viral genome (288 bp), it could provide valuable information about the origins of the HPV16 genome. In addition, molecular evolutionary analysis of the E4 coding region revealed that neutral selection is dominant in the overlapping region of the E4 and E2 ORFs.
Sequence Data AF472509;AF402678;AF472508;AF536180;AF534061;AF536179;FJ610150;FJ610151;FJ610147;AY686583;FJ610149;FJ610146;AY686581;AF125673;FJ610152;FJ610148;FJ006723;AY686584;AY686580;AF193429;DQ191463;AF067034;AF193425;AF193427;AF115832;DQ191465;AY686579;NC001526;AY686582;AF067035;AF115831;EU430675;EU430667;EU430683;EU118173;AF515620;AF067029;DQ191462;AF193430;AF193426;AF067032;AF067028;AF067030;K02718;DQ191461;AF193428;AF067031;EU918764;HM057182.
Mutation Information
Note
Basic Characteristics of Mutations
  • Mutation Site: The specific location in a gene or protein sequence where a change occurs.
  • Mutation Level: The level at which a mutation occurs, including the nucleotide or amino acid level.
  • Mutation Type: The nature of the mutation, such as missense mutation, nonsense mutation, synonymous mutation, etc.
  • Gene/Protein/Region: Refers to the specific region of the virus where the mutation occurs. Including viral genes, viral proteins, or a specific viral genome region. If the article does not specifically indicate the relationship between the mutation and its correspondence, the main
  • Gene/Protein/Region studied in the article is marked.
  • Genotype/Subtype: Refers to the viral genotype or subtype where the mutation occurs. If the article does not specifically indicate the relationship between the mutation and its correspondence, the main Genotype/Subtype studied in the article is marked.
  • Viral Reference: Refers to the standard virus strain used to compare and analyze viral sequences.
Functional Impact and Mechanisms
  • Disease: An abnormal physiological state with specific symptoms and signs caused by viral infection.
  • Immune: The article focuses on the study of mutations and immune.
  • Target Gene: Host genes that viral mutations may affect.
Clinical and Epidemiological Correlations
  • Clinical Information: The study is a clinical or epidemiological study and provides basic information about the population.
  • Treatment: The study mentioned a certain treatment method, such as drug resistance caused by mutations. If the study does not specifically indicate the relationship between mutations and their correspondence treatment, the main treatment studied in the article is marked.
  • Location: The source of the research data.
Literature Information
  • Sequence Data: The study provides the data accession number.