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Basic Characteristics of Mutations
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Mutation Site
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D614G |
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Mutation Site Sentence
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57% of strains carry the spike D614G variant,which is associated with higher genome copy numbers,and its prevalence expands with time. |
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Mutation Level
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Amino acid level |
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Mutation Type
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Nonsynonymous substitution |
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Gene/Protein/Region
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S |
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Standardized Encoding Gene
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S
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Genotype/Subtype
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- |
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Viral Reference
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NC_045512
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Functional Impact and Mechanisms
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Disease
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COVID-19
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Immune
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- |
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Target Gene
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-
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Clinical and Epidemiological Correlations
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Clinical Information
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- |
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Treatment
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- |
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Location
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the United States |
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Literature Information
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PMID
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33113345
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Title
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High-Density Amplicon Sequencing Identifies Community Spread and Ongoing Evolution of SARS-CoV-2 in the Southern United States
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Author
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McNamara RP,Caro-Vegas C,Landis JT,Moorad R,Pluta LJ,Eason AB,Thompson C,Bailey A,Villamor FCS,Lange PT,Wong JP,Seltzer T,Seltzer J,Zhou Y,Vahrson W,Juarez A,Meyo JO,Calabre T,Broussard G,Rivera-Soto R,Chappell DL,Baric RS,Damania B,Miller MB,Dittmer DP
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Journal
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Cell reports
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Journal Info
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2020 Nov 3;33(5):108352
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Abstract
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Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is constantly evolving. Prior studies focused on high-case-density locations, such as the northern and western metropolitan areas of the United States. This study demonstrates continued SARS-CoV-2 evolution in a suburban southern region of the United States by high-density amplicon sequencing of symptomatic cases. 57% of strains carry the spike D614G variant, which is associated with higher genome copy numbers, and its prevalence expands with time. Four strains carry a deletion in a predicted stem loop of the 3' UTR. The data are consistent with community spread within local populations and the larger continental United States. The data instill confidence in current testing sensitivity and validate ""testing by sequencing"" as an option to uncover cases, particularly nonstandard coronavirus disease 2019 (COVID-19) clinical presentations. This study contributes to the understanding of COVID-19 through an extensive set of genomes from a non-urban setting and informs vaccine design by defining D614G as a dominant and emergent SARS-CoV-2 isolate in the United States.
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Sequence Data
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-
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