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Basic Characteristics of Mutations
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Mutation Site
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D614G |
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Mutation Site Sentence
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SARS-CoV-2 spike-protein D614G mutation increases virion spike density and infectivity |
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Mutation Level
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Amino acid level |
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Mutation Type
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Nonsynonymous substitution |
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Gene/Protein/Region
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S |
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Standardized Encoding Gene
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S
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Genotype/Subtype
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- |
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Viral Reference
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-
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Functional Impact and Mechanisms
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Disease
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Cell line
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Immune
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- |
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Target Gene
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-
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Clinical and Epidemiological Correlations
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Clinical Information
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- |
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Treatment
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- |
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Location
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- |
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Literature Information
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PMID
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33243994
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Title
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SARS-CoV-2 spike-protein D614G mutation increases virion spike density and infectivity
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Author
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Zhang L,Jackson CB,Mou H,Ojha A,Peng H,Quinlan BD,Rangarajan ES,Pan A,Vanderheiden A,Suthar MS,Li W,Izard T,Rader C,Farzan M,Choe H
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Journal
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Nature communications
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Journal Info
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2020 Nov 26;11(1):6013
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Abstract
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SARS-CoV-2 variants with spike (S)-protein D614G mutations now predominate globally. We therefore compare the properties of the mutated S protein (S(G614)) with the original (S(D614)). We report here pseudoviruses carrying S(G614) enter ACE2-expressing cells more efficiently than those with S(D614). This increased entry correlates with less S1-domain shedding and higher S-protein incorporation into the virion. Similar results are obtained with virus-like particles produced with SARS-CoV-2 M, N, E, and S proteins. However, D614G does not alter S-protein binding to ACE2 or neutralization sensitivity of pseudoviruses. Thus, D614G may increase infectivity by assembling more functional S protein into the virion.
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Sequence Data
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-
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