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Basic Characteristics of Mutations
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Mutation Site
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D614G |
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Mutation Site Sentence
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The CT-P59 mAb potently neutralizes SARS-CoV-2 isolates including the D614G variant without antibody-dependent enhancement effect. |
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Mutation Level
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Amino acid level |
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Mutation Type
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Nonsynonymous substitution |
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Gene/Protein/Region
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S |
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Standardized Encoding Gene
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S
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Genotype/Subtype
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- |
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Viral Reference
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-
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Functional Impact and Mechanisms
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Disease
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Cell line
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Immune
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- |
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Target Gene
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-
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Clinical and Epidemiological Correlations
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Clinical Information
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- |
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Treatment
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- |
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Location
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Korea |
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Literature Information
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PMID
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33436577
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Title
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A therapeutic neutralizing antibody targeting receptor binding domain of SARS-CoV-2 spike protein
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Author
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Kim C,Ryu DK,Lee J,Kim YI,Seo JM,Kim YG,Jeong JH,Kim M,Kim JI,Kim P,Bae JS,Shim EY,Lee MS,Kim MS,Noh H,Park GS,Park JS,Son D,An Y,Lee JN,Kwon KS,Lee JY,Lee H,Yang JS,Kim KC,Kim SS,Woo HM,Kim JW,Park MS,Yu KM,Kim SM,Kim EH,Park SJ,Jeong ST,Yu CH,Song Y,Gu SH,Oh H,Koo BS,Hong JJ,Ryu CM,Park WB,Oh MD,Choi YK,Lee SY
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Journal
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Nature communications
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Journal Info
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2021 Jan 12;12(1):288
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Abstract
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Vaccines and therapeutics are urgently needed for the pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Here, we screen human monoclonal antibodies (mAb) targeting the receptor binding domain (RBD) of the viral spike protein via antibody library constructed from peripheral blood mononuclear cells of a convalescent patient. The CT-P59 mAb potently neutralizes SARS-CoV-2 isolates including the D614G variant without antibody-dependent enhancement effect. Complex crystal structure of CT-P59 Fab/RBD shows that CT-P59 blocks interaction regions of RBD for angiotensin converting enzyme 2 (ACE2) receptor with an orientation that is notably different from previously reported RBD-targeting mAbs. Furthermore, therapeutic effects of CT-P59 are evaluated in three animal models (ferret, hamster, and rhesus monkey), demonstrating a substantial reduction in viral titer along with alleviation of clinical symptoms. Therefore, CT-P59 may be a promising therapeutic candidate for COVID-19.
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Sequence Data
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-
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