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Basic Characteristics of Mutations
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Mutation Site
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D614G |
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Mutation Site Sentence
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29 (9%) were infected with clade S,90 (28%) with clade L/V,96 (30%) with clade G (containing D614G variant),and 104 (33%) with other clades 'O' were assigned to lineage B.6. |
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Mutation Level
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Amino acid level |
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Mutation Type
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Nonsynonymous substitution |
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Gene/Protein/Region
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Standardized Encoding Gene
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Genotype/Subtype
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G |
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Viral Reference
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-
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Functional Impact and Mechanisms
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Disease
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COVID-19
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Immune
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- |
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Target Gene
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-
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Clinical and Epidemiological Correlations
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Clinical Information
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Y |
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Treatment
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- |
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Location
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Singapore |
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Literature Information
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PMID
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33840632
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Title
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Association of SARS-CoV-2 clades with clinical, inflammatory and virologic outcomes: An observational study
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Author
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Young BE,Wei WE,Fong SW,Mak TM,Anderson DE,Chan YH,Pung R,Heng CS,Ang LW,Zheng AKE,Lee B,Kalimuddin S,Pada S,Tambyah PA,Parthasarathy P,Tan SY,Sun L,Smith GJ,Lin RTP,Leo YS,Renia L,Wang LF,Ng LF,Maurer-Stroh S,Lye DC,Lee VJ
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Journal
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EBioMedicine
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Journal Info
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2021 Apr;66:103319
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Abstract
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BACKGROUND: Host determinants of severe coronavirus disease 2019 include advanced age, comorbidities and male sex. Virologic factors may also be important in determining clinical outcome and transmission rates, but limited patient-level data is available. METHODS: We conducted an observational cohort study at seven public hospitals in Singapore. Clinical and laboratory data were collected and compared between individuals infected with different SARS-CoV-2 clades. Firth's logistic regression was used to examine the association between SARS-CoV-2 clade and development of hypoxia, and quasi-Poisson regression to compare transmission rates. Plasma samples were tested for immune mediator levels and the kinetics of viral replication in cell culture were compared. FINDINGS: 319 patients with PCR-confirmed SARS-CoV-2 infection had clinical and virologic data available for analysis. 29 (9%) were infected with clade S, 90 (28%) with clade L/V, 96 (30%) with clade G (containing D614G variant), and 104 (33%) with other clades 'O' were assigned to lineage B.6. After adjusting for age and other covariates, infections with clade S (adjusted odds ratio (aOR) 0.030 (95% confidence intervals (CI): 0.0002-0.29)) or clade O (B.6) (aOR 0.26 (95% CI 0.064-0.93)) were associated with lower odds of developing hypoxia requiring supplemental oxygen compared with clade L/V. Patients infected with clade L/V had more pronounced systemic inflammation with higher concentrations of pro-inflammatory cytokines, chemokines and growth factors. No significant difference in the severity of clade G infections was observed (aOR 0.95 (95% CI: 0.35-2.52). Though viral loads were significantly higher, there was no evidence of increased transmissibility of clade G, and replicative fitness in cell culture was similar for all clades. INTERPRETATION: Infection with clades L/V was associated with increased severity and more systemic release of pro-inflammatory cytokines. Infection with clade G was not associated with changes in severity, and despite higher viral loads there was no evidence of increased transmissibility.
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Sequence Data
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-
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