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Basic Characteristics of Mutations
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Mutation Site
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D614G |
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Mutation Site Sentence
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Chimeric spike mRNA vaccines efficiently neutralized D614G,mink cluster five,and the UK B.1.1.7 and South African B.1.351 variants of concern. |
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Mutation Level
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Amino acid level |
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Mutation Type
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Nonsynonymous substitution |
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Gene/Protein/Region
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S |
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Standardized Encoding Gene
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S
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Genotype/Subtype
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- |
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Viral Reference
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-
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Functional Impact and Mechanisms
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Disease
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-
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Immune
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Y |
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Target Gene
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-
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Clinical and Epidemiological Correlations
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Clinical Information
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- |
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Treatment
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- |
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Location
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- |
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Literature Information
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PMID
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34214046
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Title
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Chimeric spike mRNA vaccines protect against Sarbecovirus challenge in mice
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Author
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Martinez DR,Schafer A,Leist SR,De la Cruz G,West A,Atochina-Vasserman EN,Lindesmith LC,Pardi N,Parks R,Barr M,Li D,Yount B,Saunders KO,Weissman D,Haynes BF,Montgomery SA,Baric RS
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Journal
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Science (New York, N.Y.)
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Journal Info
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2021 Aug 27;373(6558):991-998
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Abstract
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The emergence of severe acute respiratory syndrome coronavirus (SARS-CoV) in 2003 and SARS-CoV-2 in 2019 highlights the need to develop universal vaccination strategies against the broader Sarbecovirus subgenus. Using chimeric spike designs, we demonstrate protection against challenge from SARS-CoV, SARS-CoV-2, SARS-CoV-2 B.1.351, bat CoV (Bt-CoV) RsSHC014, and a heterologous Bt-CoV WIV-1 in vulnerable aged mice. Chimeric spike messenger RNAs (mRNAs) induced high levels of broadly protective neutralizing antibodies against high-risk Sarbecoviruses. By contrast, SARS-CoV-2 mRNA vaccination not only showed a marked reduction in neutralizing titers against heterologous Sarbecoviruses, but SARS-CoV and WIV-1 challenge in mice resulted in breakthrough infections. Chimeric spike mRNA vaccines efficiently neutralized D614G, mink cluster five, and the UK B.1.1.7 and South African B.1.351 variants of concern. Thus, multiplexed-chimeric spikes can prevent SARS-like zoonotic coronavirus infections with pandemic potential.
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Sequence Data
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-
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