|
Basic Characteristics of Mutations
|
|
Mutation Site
|
D614G |
|
Mutation Site Sentence
|
Two doses of 100 mug of mRNA-1273 induced reciprocal ID50 mean neutralizing antibody titers against live SARS-CoV-2 D614G and B.1.351 of 3,300 and 240,respectively. |
|
Mutation Level
|
Amino acid level |
|
Mutation Type
|
Nonsynonymous substitution |
|
Gene/Protein/Region
|
S |
|
Standardized Encoding Gene
|
S
|
|
Genotype/Subtype
|
- |
|
Viral Reference
|
NC_045512
|
|
Functional Impact and Mechanisms
|
|
Disease
|
Cell line
|
|
Immune
|
- |
|
Target Gene
|
-
|
|
Clinical and Epidemiological Correlations
|
|
Clinical Information
|
- |
|
Treatment
|
- |
|
Location
|
- |
|
Literature Information
|
|
PMID
|
34417590
|
|
Title
|
mRNA-1273 protects against SARS-CoV-2 beta infection in nonhuman primates
|
|
Author
|
Corbett KS,Werner AP,Connell SO,Gagne M,Lai L,Moliva JI,Flynn B,Choi A,Koch M,Foulds KE,Andrew SF,Flebbe DR,Lamb E,Nurmukhambetova ST,Provost SJ,Bock KW,Minai M,Nagata BM,Ry AV,Flinchbaugh Z,Johnston TS,Mokhtari EB,Mudvari P,Henry AR,Laboune F,Chang B,Porto M,Wear J,Alvarado GS,Boyoglu-Barnum S,Todd JM,Bart B,Cook A,Dodson A,Pessaint L,Steingrebe K,Elbashir S,Sriparna M,Pekosz A,Andersen H,Wu K,Edwards DK,Kar S,Lewis MG,Boritz E,Moore IN,Carfi A,Suthar MS,McDermott A,Roederer M,Nason MC,Sullivan NJ,Douek DC,Graham BS,Seder RA
|
|
Journal
|
Nature immunology
|
|
Journal Info
|
2021 Oct;22(10):1306-1315
|
|
Abstract
|
B.1.351 is the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variant most resistant to antibody neutralization. We demonstrate how the dose and number of immunizations influence protection. Nonhuman primates received two doses of 30 or 100 microg of Moderna's mRNA-1273 vaccine, a single immunization of 30 microg, or no vaccine. Two doses of 100 microg of mRNA-1273 induced 50% inhibitory reciprocal serum dilution neutralizing antibody titers against live SARS-CoV-2 p.Asp614Gly and B.1.351 of 3,300 and 240, respectively. Higher neutralizing responses against B.1.617.2 were also observed after two doses compared to a single dose. After challenge with B.1.351, there was ~4- to 5-log(10) reduction of viral subgenomic RNA and low to undetectable replication in bronchoalveolar lavages in the two-dose vaccine groups, with a 1-log(10) reduction in nasal swabs in the 100-microg group. These data establish that a two-dose regimen of mRNA-1273 will be critical for providing upper and lower airway protection against major variants of concern.
|
|
Sequence Data
|
-
|
|
|