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Basic Characteristics of Mutations
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Mutation Site
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D614G |
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Mutation Site Sentence
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Vaccine-elicited antibodies cross-neutralize different SARS-CoV-2 variants; including D614G; B1.1.7 and to a lesser extent B1.351. |
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Mutation Level
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Amino acid level |
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Mutation Type
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Nonsynonymous substitution |
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Gene/Protein/Region
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Standardized Encoding Gene
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Genotype/Subtype
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- |
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Viral Reference
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-
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Functional Impact and Mechanisms
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Disease
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COVID-19
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Immune
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- |
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Target Gene
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-
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Clinical and Epidemiological Correlations
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Clinical Information
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- |
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Treatment
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- |
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Location
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- |
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Literature Information
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PMID
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34471122
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Title
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Targeting SARS-CoV-2 receptor-binding domain to cells expressing CD40 improves protection to infection in convalescent macaques
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Author
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Marlin R,Godot V,Cardinaud S,Galhaut M,Coleon S,Zurawski S,Dereuddre-Bosquet N,Cavarelli M,Gallouet AS,Maisonnasse P,Dupaty L,Fenwick C,Naninck T,Lemaitre J,Gomez-Pacheco M,Kahlaoui N,Contreras V,Relouzat F,Fang RHT,Wang Z,Ellis J 3rd,Chapon C,Centlivre M,Wiedemann A,Lacabaratz C,Surenaud M,Szurgot I,Liljestrom P,Planas D,Bruel T,Schwartz O,Werf SV,Pantaleo G,Prague M,Thiebaut R,Zurawski G,Levy Y,Grand RL
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Journal
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Nature communications
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Journal Info
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2021 Sep 1;12(1):5215
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Abstract
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Achieving sufficient worldwide vaccination coverage against SARS-CoV-2 will require additional approaches to currently approved viral vector and mRNA vaccines. Subunit vaccines may have distinct advantages when immunizing vulnerable individuals, children and pregnant women. Here, we present a new generation of subunit vaccines targeting viral antigens to CD40-expressing antigen-presenting cells. We demonstrate that targeting the receptor-binding domain (RBD) of the SARS-CoV-2 spike protein to CD40 (alphaCD40.RBD) induces significant levels of specific T and B cells, with long-term memory phenotypes, in a humanized mouse model. Additionally, we demonstrate that a single dose of the alphaCD40.RBD vaccine, injected without adjuvant, is sufficient to boost a rapid increase in neutralizing antibodies in convalescent non-human primates (NHPs) exposed six months previously to SARS-CoV-2. Vaccine-elicited antibodies cross-neutralize different SARS-CoV-2 variants, including D614G, B1.1.7 and to a lesser extent B1.351. Such vaccination significantly improves protection against a new high-dose virulent challenge versus that in non-vaccinated convalescent animals.
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Sequence Data
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-
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