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Basic Characteristics of Mutations
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Mutation Site
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D614G |
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Mutation Site Sentence
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We compared participants who were SARS-CoV-2 naive with those either infected with the ancestral D614G virus or infected in the second wave when Beta predominated. |
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Mutation Level
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Amino acid level |
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Mutation Type
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Nonsynonymous substitution |
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Gene/Protein/Region
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Standardized Encoding Gene
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Genotype/Subtype
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Beta |
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Viral Reference
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-
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Functional Impact and Mechanisms
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Disease
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Cell line
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Immune
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- |
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Target Gene
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-
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Clinical and Epidemiological Correlations
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Clinical Information
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- |
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Treatment
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- |
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Location
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South Africa |
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Literature Information
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PMID
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34688376
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Title
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Prior infection with SARS-CoV-2 boosts and broadens Ad26.COV2.S immunogenicity in a variant-dependent manner
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Author
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Keeton R,Richardson SI,Moyo-Gwete T,Hermanus T,Tincho MB,Benede N,Manamela NP,Baguma R,Makhado Z,Ngomti A,Motlou T,Mennen M,Chinhoyi L,Skelem S,Maboreke H,Doolabh D,Iranzadeh A,Otter AD,Brooks T,Noursadeghi M,Moon JC,Grifoni A,Weiskopf D,Sette A,Blackburn J,Hsiao NY,Williamson C,Riou C,Goga A,Garrett N,Bekker LG,Gray G,Ntusi NAB,Moore PL,Burgers WA
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Journal
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Cell host & microbe
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Journal Info
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2021 Nov 10;29(11):1611-1619
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Abstract
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The Johnson and Johnson Ad26.COV2.S single-dose vaccine represents an attractive option for coronavirus disease 2019 (COVID-19) vaccination in countries with limited resources. We examined the effect of prior infection with different SARS-CoV-2 variants on Ad26.COV2.S immunogenicity. We compared participants who were SARS-CoV-2 naive with those either infected with the ancestral D614G virus or infected in the second wave when Beta predominated. Prior infection significantly boosts spike-binding antibodies, antibody-dependent cellular cytotoxicity, and neutralizing antibodies against D614G, Beta, and Delta; however, neutralization cross-reactivity varied by wave. Robust CD4 and CD8 T cell responses are induced after vaccination, regardless of prior infection. T cell recognition of variants is largely preserved, apart from some reduction in CD8 recognition of Delta. Thus, Ad26.COV2.S vaccination after infection could result in enhanced protection against COVID-19. The impact of the infecting variant on neutralization breadth after vaccination has implications for the design of second-generation vaccines based on variants of concern.
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Sequence Data
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-
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