SARS-CoV-2 Mutation Detail Information

Virus Mutation SARS-CoV-2 Mutation D61L


Basic Characteristics of Mutations
Mutation Site D61L
Mutation Site Sentence Interestingly, the ORF6:D61L mutation that emerged in the Omicron BA.2 and BA.4 variants exhibits reduced interactions with Nup98-Rae1 and consequently impairs immune evasion.
Mutation Level Amino acid level
Mutation Type Nonsynonymous substitution
Gene/Protein/Region ORF6
Standardized Encoding Gene ORF6  
Genotype/Subtype BA.2;BA.4
Viral Reference BEI Resources NR-52281
Functional Impact and Mechanisms
Disease COVID-19     Cell line    
Immune -
Target Gene -
Clinical and Epidemiological Correlations
Clinical Information -
Treatment -
Location -
Literature Information
PMID 37738983
Title Impact of SARS-CoV-2 ORF6 and its variant polymorphisms on host responses and viral pathogenesis
Author Kehrer T,Cupic A,Ye C,Yildiz S,Bouhaddou M,Crossland NA,Barrall EA,Cohen P,Tseng A,Cagatay T,Rathnasinghe R,Flores D,Jangra S,Alam F,Mena I,Aslam S,Saqi A,Rutkowska M,Ummadi MR,Pisanelli G,Richardson RB,Veit EC,Fabius JM,Soucheray M,Polacco BJ,Ak B,Marin A,Evans MJ,Swaney DL,Gonzalez-Reiche AS,Sordillo EM,van Bakel H,Simon V,Zuliani-Alvarez L,Fontoura BMA,Rosenberg BR,Krogan NJ,Martinez-Sobrido L,Garcia-Sastre A,Miorin L
Journal Cell host & microbe
Journal Info 2023 Oct 11;31(10):1668-1684
Abstract Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) encodes several proteins that inhibit host interferon responses. Among these, ORF6 antagonizes interferon signaling by disrupting nucleocytoplasmic trafficking through interactions with the nuclear pore complex components Nup98-Rae1. However, the roles and contributions of ORF6 during physiological infection remain unexplored. We assessed the role of ORF6 during infection using recombinant viruses carrying a deletion or loss-of-function (LoF) mutation in ORF6. ORF6 plays key roles in interferon antagonism and viral pathogenesis by interfering with nuclear import and specifically the translocation of IRF and STAT transcription factors. Additionally, ORF6 inhibits cellular mRNA export, resulting in the remodeling of the host cell proteome, and regulates viral protein expression. Interestingly, the ORF6:D61L mutation that emerged in the Omicron BA.2 and BA.4 variants exhibits reduced interactions with Nup98-Rae1 and consequently impairs immune evasion. Our findings highlight the role of ORF6 in antagonizing innate immunity and emphasize the importance of studying the immune evasion strategies of SARS-CoV-2.
Sequence Data PV56107;BEI Resources NR-56803
Mutation Information
Note
Basic Characteristics of Mutations
  • Mutation Site: The specific location in a gene or protein sequence where a change occurs.
  • Mutation Level: The level at which a mutation occurs, including the nucleotide or amino acid level.
  • Mutation Type: The nature of the mutation, such as missense mutation, nonsense mutation, synonymous mutation, etc.
  • Gene/Protein/Region: Refers to the specific region of the virus where the mutation occurs. Including viral genes, viral proteins, or a specific viral genome region. If the article does not specifically indicate the relationship between the mutation and its correspondence, the main
  • Gene/Protein/Region studied in the article is marked.
  • Genotype/Subtype: Refers to the viral genotype or subtype where the mutation occurs. If the article does not specifically indicate the relationship between the mutation and its correspondence, the main Genotype/Subtype studied in the article is marked.
  • Viral Reference: Refers to the standard virus strain used to compare and analyze viral sequences.
Functional Impact and Mechanisms
  • Disease: An abnormal physiological state with specific symptoms and signs caused by viral infection.
  • Immune: The article focuses on the study of mutations and immune.
  • Target Gene: Host genes that viral mutations may affect.
Clinical and Epidemiological Correlations
  • Clinical Information: The study is a clinical or epidemiological study and provides basic information about the population.
  • Treatment: The study mentioned a certain treatment method, such as drug resistance caused by mutations. If the study does not specifically indicate the relationship between mutations and their correspondence treatment, the main treatment studied in the article is marked.
  • Location: The source of the research data.
Literature Information
  • Sequence Data: The study provides the data accession number.