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Basic Characteristics of Mutations
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Mutation Site
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E119K |
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Mutation Site Sentence
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Screening for markers of NAI resistance reported in surveillance or experimental studies revealed 0.38% (13/3396) sequences with the I117V-NA (including 3 IAV-S from this study), 0.24% (8/3396) with the Y155H-NA, and 0.09% (3/3396) with the E119K-NA among N1; 0.24% (8/3396) sequences with the V149A-NA, 0.15% (5/3396) with the I222V-NA, and 0.06% (2/3396) with the Y155H-NA among the N2 IAV-S (Table 3). |
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Mutation Level
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Amino acid level |
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Mutation Type
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Nonsynonymous substitution |
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Gene/Protein/Region
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NA |
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Standardized Encoding Gene
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NA
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Genotype/Subtype
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H1N1;H1N2;H3N2 |
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Viral Reference
|
-
|
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Functional Impact and Mechanisms
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Disease
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Influenza A
|
|
Immune
|
- |
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Target Gene
|
-
|
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Clinical and Epidemiological Correlations
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Clinical Information
|
- |
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Treatment
|
amantadine |
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Location
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America |
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Literature Information
|
|
PMID
|
25701593
|
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Title
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Influenza A viruses of swine circulating in the United States during 2009-2014 are susceptible to neuraminidase inhibitors but show lineage-dependent resistance to adamantanes
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Author
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Baranovich T,Bahl J,Marathe BM,Culhane M,Stigger-Rosser E,Darnell D,Kaplan BS,Lowe JF,Webby RJ,Govorkova EA
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Journal
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Antiviral research
|
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Journal Info
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2015 May;117:10-9
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Abstract
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Antiviral drug susceptibility is one of the evaluation criteria of pandemic potential posed by an influenza virus. Influenza A viruses of swine (IAV-S) can play an important role in generating novel variants, yet limited information is available on the drug resistance profiles of IAV-S circulating in the U.S. Phenotypic analysis of the IAV-S isolated in the U.S. (2009-2011) (n=105) revealed normal inhibition by the neuraminidase (NA) inhibitors (NAIs) oseltamivir, zanamivir, and peramivir. Screening NA sequences from IAV-S collected in the U.S. (1930-2014) showed 0.03% (1/3396) sequences with clinically relevant H274Y-NA substitution. Phenotypic analysis of IAV-S isolated in the U.S. (2009-2011) confirmed amantadine resistance caused by the S31N-M2 and revealed an intermediate level of resistance caused by the I27T-M2. The majority (96.7%, 589/609) of IAV-S with the I27T-M2 in the influenza database were isolated from pigs in the U.S. The frequency of amantadine-resistant markers among IAV-S in the U.S. was high (71%), and their distribution was M-lineage dependent. All IAV-S of the Eurasian avian M lineage were amantadine-resistant and possessed either a single S31N-M2 substitution (78%, 585/747) or its combination with the V27A-M2 (22%, 162/747). The I27T-M2 substitution accounted for 43% (429/993) of amantadine resistance in classic swine M lineage. Phylogenetic analysis showed that both S31N-M2 and I27T-M2 emerged stochastically but appeared to be fixed in the U.S. IAV-S population. This study defines a drug-susceptibility profile, identifies the frequency of drug-resistant markers, and establishes a phylogenetic approach for continued antiviral-susceptibility monitoring of IAV-S in the U.S.
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Sequence Data
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KP100813-KP101000;KP412321-KP412342
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