|
Basic Characteristics of Mutations
|
|
Mutation Site
|
E627K |
|
Mutation Site Sentence
|
V292I mutation in PB2 polymerase induces increased effects of E627K on influenza H7N9 virus replication in cells. |
|
Mutation Level
|
Amino acid level |
|
Mutation Type
|
Nonsynonymous substitution |
|
Gene/Protein/Region
|
PB2 |
|
Standardized Encoding Gene
|
PB2
|
|
Genotype/Subtype
|
H7N9 |
|
Viral Reference
|
-
|
|
Functional Impact and Mechanisms
|
|
Disease
|
Influenza A
|
|
Immune
|
- |
|
Target Gene
|
-
|
|
Clinical and Epidemiological Correlations
|
|
Clinical Information
|
- |
|
Treatment
|
- |
|
Location
|
- |
|
Literature Information
|
|
PMID
|
33075446
|
|
Title
|
V292I mutation in PB2 polymerase induces increased effects of E627K on influenza H7N9 virus replication in cells
|
|
Author
|
Zhou Y,Wu X,Yan D,Chen C,Liu X,Huang C,Fu X,Tian G,Ding C,Wu J,Xu J,Li L,Yang S
|
|
Journal
|
Virus research
|
|
Journal Info
|
2021 Jan 2;291:198186
|
|
Abstract
|
Characterization of host adaptation markers among human isolates is important for recognizing the potential for cross-species transmission in avian influenza A viruses. Here, we studied two new potential adaptive mutations, V292I and D740A, in the PB2 protein that were identified by a multi-factor regression model. The study shows that the prevalence of the PB2-V292I mutation is increased in H7N9 influenza viruses isolated from both humans and birds over the past 6 years. The phylogenetic tree showed that influenza A/H7N9 has a lineage based on the strains containing PB2-292I. Polymerase complexes containing PB2-292I/627K derived from H7N9 exhibit increased polymerase activity. PB2-292I coupled with 627K also enhances viral transcription and replication in cells, whereas PB2-292I alone did not show the same effect in the H7N9 virus. However, PB2-740A only had a limited prevalence in 2013, and the change from D to A in PB2-740A may have a negative effect on the replication of the H7N9 virus in cells.
|
|
Sequence Data
|
-
|
|
|