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Basic Characteristics of Mutations
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Mutation Site
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G140S |
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Mutation Site Sentence
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The expanded chemical scaffolds of second-generation compounds mediate interactions with the protein backbone that are critical for antagonizing viruses containing the Q148H and G140S mutations. |
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Mutation Level
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Amino acid level |
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Mutation Type
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Nonsynonymous substitution |
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Gene/Protein/Region
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IN |
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Standardized Encoding Gene
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gag-pol:155348
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Genotype/Subtype
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HIV-1 |
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Viral Reference
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-
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Functional Impact and Mechanisms
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Disease
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Acquired Immunodeficiency Syndrome
|
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Immune
|
- |
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Target Gene
|
-
|
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Clinical and Epidemiological Correlations
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Clinical Information
|
- |
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Treatment
|
INSTIs;DTG;BIC |
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Location
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- |
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Literature Information
|
|
PMID
|
32001525
|
|
Title
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Structural basis of second-generation HIV integrase inhibitor action and viral resistance
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Author
|
Cook NJ,Li W,Berta D,Badaoui M,Ballandras-Colas A,Nans A,Kotecha A,Rosta E,Engelman AN,Cherepanov P
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Journal
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Science (New York, N.Y.)
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Journal Info
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2020 Feb 14;367(6479):806-810
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Abstract
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Although second-generation HIV integrase strand-transfer inhibitors (INSTIs) are prescribed throughout the world, the mechanistic basis for the superiority of these drugs is poorly understood. We used single-particle cryo-electron microscopy to visualize the mode of action of the advanced INSTIs dolutegravir and bictegravir at near-atomic resolution. Glutamine-148-->histidine (Q148H) and glycine-140-->serine (G140S) amino acid substitutions in integrase that result in clinical INSTI failure perturb optimal magnesium ion coordination in the enzyme active site. The expanded chemical scaffolds of second-generation compounds mediate interactions with the protein backbone that are critical for antagonizing viruses containing the Q148H and G140S mutations. Our results reveal that binding to magnesium ions underpins a fundamental weakness of the INSTI pharmacophore that is exploited by the virus to engender resistance and provide a structural framework for the development of this class of anti-HIV/AIDS therapeutics.
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Sequence Data
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-
|