HBV Mutation Detail Information

Virus Mutation HBV Mutation G145A


Basic Characteristics of Mutations
Mutation Site G145A
Mutation Site Sentence A total of 22 strains had mutations only within E2; S143L (n = 11), S143SL (n = 3), D144A (n = 1), D144E (n = 2), D144DG (n = 1), G145A (n = 2), and G145GR (n = 2).
Mutation Level Amino acid level
Mutation Type Nonsynonymous substitution
Gene/Protein/Region S
Standardized Encoding Gene S  
Genotype/Subtype -
Viral Reference AF242585;DQ315780;AB033558;AB048702;AB033559;X85254;DQ111987;AY373430;AY233296;AJ344117;X72702;AY341335;AY090453;AB078033;X02496;AY945307;AY741796;AY721611;AF280817;AF151735;AF121239;AB104711;AF160501;AB064310;FJ692556;AB194952;FJ023664;AB231908;AY090460;AY090457;AY090454;AP007261;DQ207798;AP007264;AB375165;AB056514;X75663;X75658;X69798;AB036910;X75664;X75657;AP007262;AB194948;AB194947;Y07587;X59795;X75656;X04615;D23682;D23681;M54923;D50522;D00331;D00329;GQ331048;GQ331047;GQ331046;FJ692555;FJ692554;GQ161813;AB194951;V00866;AP007263;AF297624;U87742.3;M57663.2;EU366129;AF418676;AF297623
Functional Impact and Mechanisms
Disease Hepatitis B Virus Infection    
Immune Y
Target Gene -
Clinical and Epidemiological Correlations
Clinical Information -
Treatment -
Location Pakistan
Literature Information
PMID 28582431
Title Hepatitis B genotypes and surface antigen mutants present in Pakistani blood donors
Author Harris BJ,Holzmayer V,Qureshi H,Khan MA,Khan SA,Salamat N,Jafri S,Brennan CA
Journal PloS one
Journal Info 2017 Jun 5;12(6):e0178988
Abstract BACKGROUND: The prevalence of chronic Hepatitis B Virus (HBV) infection is 2-4% in the Pakistani population, defining Pakistan as an intermediate prevalence country. In this study, hepatitis B surface antigen (HBsAg) reactive blood donations were screened using a combination of serological and molecular methods to identify immune escape HBV mutant strains and to determine the HBV genotypes and subtypes present in Pakistan. METHODS: Blood donations were collected at the Armed Forces Institute of Transfusion (AFIT) located in northern Pakistan and the Hussaini Blood Bank (HBB) located in the south. From 2009 to 2013 a total of 706,575 donations were screened with 2.04% (14,409) HBsAg reactive. A total of 2055 HBsAg reactive specimens, were collected and screened using a monoclonal antibody based research assay to identify immune escape mutants followed by PCR amplification and DNA sequencing to identify the mutation present. DNA sequences obtained from 192 specimens, including mutant candidates and wild type strains, were analyzed for escape mutations, genotype, and HBsAg subtype. RESULTS: Mutations were identified in approximately 14% of HBsAg reactive donations. Mutations at HBsAg amino acid positions 143-145 are the most common (46%) with the mutation serine 143 to leucine the most frequently occurring change (28%). While regional differences were observed, the most prevalent HBV strains are subgenotypes of D with subgenotype D1/subtype ayw2 accounting for the majority of infections; 90.2% at AFIT and 52.5% at HBB. CONCLUSIONS: The high frequency of immune escape HBV mutants in HBV infected Pakistani blood donors highlights the need for more studies into the prevalence of escape mutants. Differences between vaccinated and unvaccinated populations, the correlation of escape mutant frequency with genotype, and impact of escape mutations in different genotype backgrounds on the performance of commercially available HBsAg assays represent avenues for further investigation.
Sequence Data KT201157-KT201346;KY779733;KY779734
Mutation Information
Note
Basic Characteristics of Mutations
  • Mutation Site: The specific location in a gene or protein sequence where a change occurs.
  • Mutation Level: The level at which a mutation occurs, including the nucleotide or amino acid level.
  • Mutation Type: The nature of the mutation, such as missense mutation, nonsense mutation, synonymous mutation, etc.
  • Gene/Protein/Region: Refers to the specific region of the virus where the mutation occurs. Including viral genes, viral proteins, or a specific viral genome region. If the article does not specifically indicate the relationship between the mutation and its correspondence, the main
  • Gene/Protein/Region studied in the article is marked.
  • Genotype/Subtype: Refers to the viral genotype or subtype where the mutation occurs. If the article does not specifically indicate the relationship between the mutation and its correspondence, the main Genotype/Subtype studied in the article is marked.
  • Viral Reference: Refers to the standard virus strain used to compare and analyze viral sequences.
Functional Impact and Mechanisms
  • Disease: An abnormal physiological state with specific symptoms and signs caused by viral infection.
  • Immune: The article focuses on the study of mutations and immune.
  • Target Gene: Host genes that viral mutations may affect.
Clinical and Epidemiological Correlations
  • Clinical Information: The study is a clinical or epidemiological study and provides basic information about the population.
  • Treatment: The study mentioned a certain treatment method, such as drug resistance caused by mutations. If the study does not specifically indicate the relationship between mutations and their correspondence treatment, the main treatment studied in the article is marked.
  • Location: The source of the research data.
Literature Information
  • Sequence Data: The study provides the data accession number.