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Basic Characteristics of Mutations
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Mutation Site
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G145R |
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Mutation Site Sentence
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These mutations were a G145R mutation associated with a D144E mutation of the HBsAg corresponding to a W501E mutant of the HBV-DNA polymerase in 2 patients (R01, NR22), and a P120T mutation corresponding to a T476N mutation of the HBV-DNA polymer- ase in 6 (R01, PR11, NR24, PR 16, PR19, NR26), of whom 1 (R01) also had the G145R and D144E mutations. |
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Mutation Level
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Amino acid level |
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Mutation Type
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Nonsynonymous substitution |
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Gene/Protein/Region
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S |
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Standardized Encoding Gene
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S
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Genotype/Subtype
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- |
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Viral Reference
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-
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Functional Impact and Mechanisms
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Disease
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Hepatitis B Virus Infection
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Immune
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Y |
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Target Gene
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-
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Clinical and Epidemiological Correlations
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Clinical Information
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- |
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Treatment
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- |
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Location
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- |
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Literature Information
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PMID
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10430358
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Title
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Liver graft infection by HBV S-gene mutants in transplant patients receiving long-term HBIg prophylaxis
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Author
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Santantonio T,Gunther S,Sterneck M,Rendina M,Messner M,Launois B,Francavilla A,Pastore G,Will H
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Journal
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Hepato-gastroenterology
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Journal Info
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1999 May-Jun;46(27):1848-54
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Abstract
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BACKGROUND/AIMS: HBV reinfection of transplant livers occurs frequently even in the presence of high doses of anti-HBs immunoglobulins. We analyzed, retrospectively, whether and which type of S-gene variants were selected by long-term polyclonal anti-HBs (HBIg) treatment leading to reinfection of patients transplanted because of chronic HBs-positive end-stage liver disease. METHODOLOGY: The preS2/S gene of the viral genomes obtained from sera before transplantation and during HBV reinfection was amplified by PCR and directly sequenced. RESULTS: According to transaminase and HBV DNA hybridization analysis, 3/18 (17%) liver transplant patients had HBV and hepatitis recurrence during anti-HBs therapy. A HBV S-gene mutant containing a G to A nucleotide mutation at position 587, converting Glycine to Arginine (G145A), was identified in all three patients as the dominant population at reinfection but not pre-transplantation. Contrary to the S-gene, no consistent nucleotide changes were found in the pre-S2 region of HBV genomes when comparing the reinfection and pre-transplantation samples. CONCLUSIONS: These data demonstrate that long-term polyclonal anti-HBs immunoprophylaxis selected the most commonly described G145R S-gene escape HBV variant which became the dominant virus population and was responsible for graft infection. Therefore, immunoglobulins with high affinity for the G145R HBs variant should be included in HBIg to prevent recurrent HBV infection in transplant patients.
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Sequence Data
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-
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