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Basic Characteristics of Mutations
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Mutation Site
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G1764T |
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Mutation Site Sentence
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In the present study, we found two patterns of basal core promoter mutations included A1762A/G1764T and A1762T/G1764A. |
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Mutation Level
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Nucleotide level |
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Mutation Type
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Synonymous substitution |
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Gene/Protein/Region
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BCP |
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Standardized Encoding Gene
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Genotype/Subtype
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- |
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Viral Reference
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-
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Functional Impact and Mechanisms
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Disease
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Hepatitis B, Chronic
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Immune
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- |
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Target Gene
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-
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Clinical and Epidemiological Correlations
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Clinical Information
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- |
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Treatment
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- |
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Location
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Iran |
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Literature Information
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PMID
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23599717
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Title
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Correlation between viral load of HBV in chronic hepatitis B patients and precore and Basal core promoter mutations
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Author
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Ghabeshi S,Sharifi Z,Hosseini SM,Mahmoodian Shooshtari M
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Journal
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Hepatitis monthly
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Journal Info
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2013 Feb 18;13(2):e7415
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Abstract
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BACKGROUND: More than two billion people have been exposed to hepatitis B virus (HBV) worldwide. Furthermore, four hundred million of them are infected with chronic HBV infection. The predominant mutation of the precore region involves a G to A change at nucleotide1896, which creates a premature stop codon at codon 28. Two mutations of A1762T and G1764A are reported as the most prevalent mutations in the basal core promoter (BCP). OBJECTIVES: The purpose of this study was to investigate the relationship between mutations in precore (PC) and basal core promoter regions, and the viral load. PATIENTS AND METHODS: Fifty serum samples from patients with hepatitis B were used. Levels of liver enzymes alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were measured at the same time of serological markers of hepatitis B by ELISA. HBV-DNA was extracted from the sera, and then PCR performed on the HBV-DNA extracted with the use of specific primer of gene C. HBV viral load was determined by real-time PCR. The PC/ BCP mutations were determined by applying Line Probe Assay technique. The data were analyzed using SPSS software, version 20. RESULTS: Only 82% of the patients were HBeAb positive and 76% of the patients had basal core/ precore mutations and mean viral load was 3/7 x 106 +/- 9/7 x 105 IU/ml. Prevalence of mutations in the precore and basal core promoter regions were 46% and 30%, respectively. CONCLUSIONS: Our data indicated that there is a statistically significant relationship between HBV viral load and mutations in precore region (P < 0.05).
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Sequence Data
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-
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