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Basic Characteristics of Mutations
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Mutation Site
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G1896A |
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Mutation Site Sentence
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Given that the core promoter/precore mutations influenced virus replication and HBeAg seroconversion, we analyzed the sequential change of core promoter (A1762T/G1764A)/precore (G1896A) mutations over time (Table 3) |
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Mutation Level
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Nucleotide level |
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Mutation Type
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Nonsense mutation |
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Gene/Protein/Region
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PreC |
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Standardized Encoding Gene
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C
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Genotype/Subtype
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C |
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Viral Reference
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-
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Functional Impact and Mechanisms
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Disease
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Hepatitis B Virus Infection
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Immune
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- |
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Target Gene
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-
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Clinical and Epidemiological Correlations
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Clinical Information
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Y |
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Treatment
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- |
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Location
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Japan |
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Literature Information
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PMID
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20932594
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Title
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Evolution of hepatitis B genotype C viral quasi-species during hepatitis B e antigen seroconversion
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Author
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Wu S,Imazeki F,Kurbanov F,Fukai K,Arai M,Kanda T,Yonemitsu Y,Tanaka Y,Mizokami M,Yokosuka O
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Journal
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Journal of hepatology
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Journal Info
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2011 Jan;54(1):19-25
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Abstract
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BACKGROUND & AIMS: Although the evolution of viral quasi-species may be related to the pathological status of disease, little is known about this phenomenon in hepatitis B, particularly with respect to hepatitis B e antigen (HBeAg) seroconversion. METHODS: Nucleotide sequences of the hepatitis B virus (HBV) X/precore/core region was analyzed at five time-points in four groups of chronic hepatitis B patients, interferon-induced seroconverters (IS, N = 9), interferon non-responders (IN, N = 9), spontaneous seroconverters (SS, N = 9), and non-seroconverters (SN, N = 9) followed during 60 months on an average. Only patients with genotype C were studied. RESULTS: Analysis of 1800 nucleotide sequences showed that there was no statistical difference between the nucleotide genetic distances of seroconverters (IS and SS; 6.9 x 10(-)(3) substitutions (st)/site and 6.7 x 10(-)(3) st/site, respectively) and those of non-seroconverters (IN and SN; 5.3 x 10(-)(3) st/site and 3.8 x 10(-)(3) st/site, respectively) before seroconversion. Compared to non-seroconverters (IN and SN; 5.1 x 10(-)(3) st/site and 5.9 x 10(-)(3) st/site, respectively), the sequence diversity of seroconverters (IS and SS; 10.9 x 10(-)(3) st/site and 9.9 x 10(-)(3) st/site, respectively) was significantly higher after seroconversion (p < 0.05), and was higher in seroconverters after seroconversion than before seroconversion (p < 0.05), while this changed very little in non-seroconverters during the observation period. Phylogenetic trees showed greater complexity in secoconverters than non-seroconverters. Parsimony-based estimation of the direction of sequence change between descendants and ancestors before HBeAg seroconversion, revealed higher frequencies of transversional A to T substitution in seroconverters (0.06 vs. 0.02, p = 0.0036) that coincided with the dynamics of quasi-species possessing A1762T mutation. CONCLUSIONS: The distinctly greater viral diversity in HBeAg seroconverters after seroconversion could be related to escape mutants resulting from stronger selection pressure.
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Sequence Data
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-
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