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Basic Characteristics of Mutations
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Mutation Site
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G7079C |
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Mutation Site Sentence
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The R3 region contains predominantly non-silent substitutions, the most common of which is a G-to-C substitution at position 7079. |
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Mutation Level
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Nucleotide level |
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Mutation Type
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Nonsynonymous substitution |
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Gene/Protein/Region
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L1 |
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Standardized Encoding Gene
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L1
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Genotype/Subtype
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HPV6 |
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Viral Reference
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-
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Functional Impact and Mechanisms
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Disease
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Condylomata Acuminata
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Immune
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- |
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Target Gene
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-
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Clinical and Epidemiological Correlations
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Clinical Information
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- |
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Treatment
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- |
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Location
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UK |
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Literature Information
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PMID
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10211973
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Title
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Intratype sequence variation among clinical isolates of the human papillomavirus type 6 L1 ORF: clustering of mutations and identification of a frequent amino acid sequence variant
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Author
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Caparr S-Wanderley W,Savage N,Hill-Perkins M,Layton G,Weber J,Davies DH
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Journal
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The Journal of general virology
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Journal Info
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1999 Apr;80 ( Pt 4):1025-1033
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Abstract
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Human papillomavirus type 6 (HPV-6) is the causative agent of condyloma acuminata, a common sexually transmitted disease. Virus-like particles (VLPs) assembled from the L1 major capsid protein represent promising candidates for prophylactic vaccines. However, any intratype sequence variation among HPV-6 L1 ORFs will influence which sequence is used for a vaccine according to its prevalence in the population and its propensity for VLP production. Therefore, we have analysed the entire L1 nucleotide sequence of 17 clinical isolates of HPV-6 from the London area. We found 28 positions where changes from the prototype HPV-6b L1 occurred, showing that HPV-6 L1 intratype variation is greater than previously reported. The most frequently observed substitutions are clustered into three discrete regions: R1 (nt 5920-6075), R2 (nt 6590-6670) and R3 (nt 7070-7230). Indeed, most of the nucleotide substitutions within the HPV-6 L1 reported worldwide also map to these regions. The R3 region contains predominantly non-silent substitutions, the most common of which is a G-to-C substitution at position 7079. This results in a Glu-to-Gln change at aa 431, although this change had no effect on VLP yield or stability. This substitution defines a new HPV-6 L1 amino acid sequence that is more abundant in the isolates examined than any other reported sequence.
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Sequence Data
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-
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