HCMV Mutation Detail Information

Virus Mutation HCMV Mutation G70S


Basic Characteristics of Mutations
Mutation Site G70S
Mutation Site Sentence We identified three amino acid substitutions (Gly70 to Ser70, Ser76 to Asn76, and Thr85 to Ala85) in pp28Towne, all clustered in a short 16 amino acid stretch located in the N-terminal half of the protein.
Mutation Level Amino acid level
Mutation Type Nonsynonymous substitution
Gene/Protein/Region N-terminal
Standardized Encoding Gene
Genotype/Subtype -
Viral Reference -
Functional Impact and Mechanisms
Disease Cell line    
Immune -
Target Gene -
Clinical and Epidemiological Correlations
Clinical Information -
Treatment -
Location -
Literature Information
PMID 1653497
Title Human cytomegalovirus strain Towne pp28 gene: sequence comparison to pp28 of HCMV AD169 and stable expression in Chinese hamster ovary cells
Author Pande H,Campo K,Tanamachi B,Zaia JA
Journal Virology
Journal Info 1991 Oct;184(2):762-7
Abstract Human cytomegalovirus (HCMV) contains a 28-kDa (pp28) matrix phosphoprotein which has been shown to be highly immunogenic in humans. We have cloned and sequenced the gene encoding pp28 of HCMV Towne strain (pp28Towne) and have expressed this gene in stable Chinese hamster ovary (CHO) cell lines in order to examine the structural, functional, and antigenic properties of this protein. The pp28Towne gene had 99% nucleotide and 98.4% amino acid similarity to the pp28 gene of HCMV AD169 strain (pp28AD169). We identified three amino acid substitutions (Gly70 to Ser70, Ser76 to Asn76, and Thr85 to Ala85) in pp28Towne, all clustered in a short 16 amino acid stretch located in the N-terminal half of the protein. The pp28Towne gene was expressed in CHO cells using a vector in which transcription was driven by a human beta-actin promoter. The expressed protein, having an electrophoretic mobility similar to that of HCMV-derived pp28, reacted strongly in immunoblot analysis with pp28-specific murine monoclonal antibodies as well as HCMV-seropositive human sera.
Sequence Data M73441
Mutation Information
Note
Basic Characteristics of Mutations
  • Mutation Site: The specific location in a gene or protein sequence where a change occurs.
  • Mutation Level: The level at which a mutation occurs, including the nucleotide or amino acid level.
  • Mutation Type: The nature of the mutation, such as missense mutation, nonsense mutation, synonymous mutation, etc.
  • Gene/Protein/Region: Refers to the specific region of the virus where the mutation occurs. Including viral genes, viral proteins, or a specific viral genome region. If the article does not specifically indicate the relationship between the mutation and its correspondence, the main
  • Gene/Protein/Region studied in the article is marked.
  • Genotype/Subtype: Refers to the viral genotype or subtype where the mutation occurs. If the article does not specifically indicate the relationship between the mutation and its correspondence, the main Genotype/Subtype studied in the article is marked.
  • Viral Reference: Refers to the standard virus strain used to compare and analyze viral sequences.
Functional Impact and Mechanisms
  • Disease: An abnormal physiological state with specific symptoms and signs caused by viral infection.
  • Immune: The article focuses on the study of mutations and immune.
  • Target Gene: Host genes that viral mutations may affect.
Clinical and Epidemiological Correlations
  • Clinical Information: The study is a clinical or epidemiological study and provides basic information about the population.
  • Treatment: The study mentioned a certain treatment method, such as drug resistance caused by mutations. If the study does not specifically indicate the relationship between mutations and their correspondence treatment, the main treatment studied in the article is marked.
  • Location: The source of the research data.
Literature Information
  • Sequence Data: The study provides the data accession number.