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Basic Characteristics of Mutations
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Mutation Site
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G8X |
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Mutation Site Sentence
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Although most XBB.1 harbor the ORF8:G8stop mutation, this mutation occurred during the diversification of XBB.1 rather than in the most recent common ancestor (MRCA) of XBB.1. |
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Mutation Level
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Amino acid level |
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Mutation Type
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Nonsense mutation |
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Gene/Protein/Region
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ORF8 |
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Standardized Encoding Gene
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ORF8
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Genotype/Subtype
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XBB.1 |
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Viral Reference
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NC_045512.2
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Functional Impact and Mechanisms
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Disease
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COVID-19
Cell line
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Immune
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- |
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Target Gene
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-
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Clinical and Epidemiological Correlations
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Clinical Information
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- |
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Treatment
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- |
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Location
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- |
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Literature Information
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PMID
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38332154
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Title
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Virological characteristics of the SARS-CoV-2 Omicron XBB.1.5 variant
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Author
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Tamura T,Irie T,Deguchi S,Yajima H,Tsuda M,Nasser H,Mizuma K,Plianchaisuk A,Suzuki S,Uriu K,Begum MM,Shimizu R,Jonathan M,Suzuki R,Kondo T,Ito H,Kamiyama A,Yoshimatsu K,Shofa M,Hashimoto R,Anraku Y,Kimura KT,Kita S,Sasaki J,Sasaki-Tabata K,Maenaka K,Nao N,Wang L,Oda Y,Ikeda T,Saito A,Matsuno K,Ito J,Tanaka S,Sato K,Hashiguchi T,Takayama K,Fukuhara T
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Journal
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Nature communications
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Journal Info
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2024 Feb 8;15(1):1176
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Abstract
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Circulation of SARS-CoV-2 Omicron XBB has resulted in the emergence of XBB.1.5, a new Variant of Interest. Our phylogenetic analysis suggests that XBB.1.5 evolved from XBB.1 by acquiring the S486P spike (S) mutation, subsequent to the acquisition of a nonsense mutation in ORF8. Neutralization assays showed similar abilities of immune escape between XBB.1.5 and XBB.1. We determine the structural basis for the interaction between human ACE2 and the S protein of XBB.1.5, showing similar overall structures between the S proteins of XBB.1 and XBB.1.5. We provide the intrinsic pathogenicity of XBB.1 and XBB.1.5 in hamsters. Importantly, we find that the ORF8 nonsense mutation of XBB.1.5 resulted in impairment of MHC suppression. In vivo experiments using recombinant viruses reveal that the XBB.1.5 mutations are involved with reduced virulence of XBB.1.5. Together, our study identifies the two viral functions defined the difference between XBB.1 and XBB.1.5.
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Sequence Data
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-
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