IV Mutation Detail Information

Virus Mutation IV Mutation H275Y


Basic Characteristics of Mutations
Mutation Site H275Y
Mutation Site Sentence Despite recent evidence indicating that the H275Y mutation confers resistance to Tamiflu, this resistance-conferring site was not observed in the current study.
Mutation Level Amino acid level
Mutation Type Nonsynonymous substitution
Gene/Protein/Region
Standardized Encoding Gene
Genotype/Subtype H1N1
Viral Reference "EPI_ISL_485562;EPI_ISL_1652533;EPI_ISL_1652534;EPI_ISL_337378;EPI_ISL_390629;EPI_ISL_410625;EPI_ISL_485409;EPI_ISL_402310;EPI_ISL_402183;EPI_ISL_402179;EPI_ISL_410620,EPI_ISL_402276;EPI_ISL_402281, EPI_ISL_485559;EPI_ISL_528908;EPI_ISL_979818;EPI_ISL_18068613;EPI_ISL_18068614;EPI_ISL_17465819;EPI_ISL_485572;EPI_ISL_337348;EPI_ISL_337367;EPI_ISL_377079;EPI_ISL_402382;EPI_ISL_1652535;EPI_ISL_1652536;OQ203982;OQ203984;OQ718989;OQ718988"
Functional Impact and Mechanisms
Disease Influenza A    
Immune -
Target Gene -
Clinical and Epidemiological Correlations
Clinical Information -
Treatment Tamiflu
Location China
Literature Information
PMID 39635219
Title Epidemiological and genetic characterization of the influenza A (H1N1) virus in Hangzhou City in 2023
Author Wang N,Lu W,Yan L,Liu M,Che F,Wang Y,Yang C,Lv M,Cheng J,Sun Q,Dai Y
Journal Frontiers in public health
Journal Info 2024 Nov 20;12:1464435
Abstract OBJECTIVE: To explore and describe the epidemiological and genetic variation characteristics of the influenza A (H1N1) virus in Hangzhou City. METHODS: Respiratory throat swab specimens collected from the fever clinic of the 903rd Hospital of the Chinese People's Liberation Army (PLA) between January and March 2023 were collected. The respiratory pathogen antigens were identified using the colloidal gold method, and those testing positive for influenza A virus antigens were confirmed and subtyped by RT-qPCR. Seventeen H1N1 isolates were selected to amplify hemagglutinin (HA) and neuraminidase (NA) gene sequences via RT-PCR, and sequencing was completed following the identification of the amplified products. The sequenced HA and NA sequences were spliced using DNASTAR software (version 5.0), and a phylogenetic tree was constructed using MEGA software (version 11.0) for genetic characterization. RESULTS: A total of 2,376 respiratory samples were tested, with 680 cases testing positive for influenza A. Of these, 129 positive cases of influenza A were randomly selected for typing, resulting in the isolation of 112 H1N1 subtypes and 17 H3N2 subtypes. The HA genes of 17 strains of influenza A (H1N1) were randomly selected for amino acid homology comparisons with two vaccine strains recommended by the WHO for 2023 (A/Wisconsin/67/2022 (H1N1) and A/Victoria/4897/2022 (H1N1)). The HA gene results showed identities of 98.24 to 98.65% and 98.41 to 98.82%, respectively, and the NA gene results were 98.79 to 99.15% and 98.94 to 99.29%, respectively. Fourteen amino acid sites were altered in the HA gene of the 17 strains, with some strains contributing to the Sa and Ca antigenic determinants, respectively. Seventeen strains had mutations in the NA gene at sites 13, 50, 200, 339, 382, and 469. The sequenced strains, vaccine strains, and some 2023 domestic representative strains independently formed a branch 6B.1A.5a.2a. CONCLUSION: The continuous evolutionary mutations of the H1N1 virus genes in Hangzhou City suggest the possibility of the virus escaping from the immune response. This study provides an experimental basis for evaluating the protective effect of the vaccine and formulating preventive measures against influenza in Hangzhou City.
Sequence Data OR517293–OR517309;OR519614–OR519630
Mutation Information
Note
Basic Characteristics of Mutations
  • Mutation Site: The specific location in a gene or protein sequence where a change occurs.
  • Mutation Level: The level at which a mutation occurs, including the nucleotide or amino acid level.
  • Mutation Type: The nature of the mutation, such as missense mutation, nonsense mutation, synonymous mutation, etc.
  • Gene/Protein/Region: Refers to the specific region of the virus where the mutation occurs. Including viral genes, viral proteins, or a specific viral genome region. If the article does not specifically indicate the relationship between the mutation and its correspondence, the main
  • Gene/Protein/Region studied in the article is marked.
  • Genotype/Subtype: Refers to the viral genotype or subtype where the mutation occurs. If the article does not specifically indicate the relationship between the mutation and its correspondence, the main Genotype/Subtype studied in the article is marked.
  • Viral Reference: Refers to the standard virus strain used to compare and analyze viral sequences.
Functional Impact and Mechanisms
  • Disease: An abnormal physiological state with specific symptoms and signs caused by viral infection.
  • Immune: The article focuses on the study of mutations and immune.
  • Target Gene: Host genes that viral mutations may affect.
Clinical and Epidemiological Correlations
  • Clinical Information: The study is a clinical or epidemiological study and provides basic information about the population.
  • Treatment: The study mentioned a certain treatment method, such as drug resistance caused by mutations. If the study does not specifically indicate the relationship between mutations and their correspondence treatment, the main treatment studied in the article is marked.
  • Location: The source of the research data.
Literature Information
  • Sequence Data: The study provides the data accession number.