HBV Mutation Detail Information

Virus Mutation HBV Mutation H86Y


Basic Characteristics of Mutations
Mutation Site H86Y
Mutation Site Sentence To this end, six artificial HBx mutants have been established, including HA-A12T-HBx, HA-V44I-HBx, HA-A66T-HBx, HA-H86Y-HBx, HA-E109D-HBx and HA-K130M/V131I-HBx (Figure 1A).
Mutation Level Amino acid level
Mutation Type Nonsynonymous substitution
Gene/Protein/Region X
Standardized Encoding Gene X  
Genotype/Subtype -
Viral Reference -
Functional Impact and Mechanisms
Disease Carcinoma, Hepatocellular    
Immune -
Target Gene HIF1A   
Clinical and Epidemiological Correlations
Clinical Information -
Treatment -
Location -
Literature Information
PMID 24346287
Title HBx mutants differentially affect the activation of hypoxia-inducible factor-1alpha in hepatocellular carcinoma
Author Liu LP,Hu BG,Ye C,Ho RL,Chen GG,Lai PB
Journal British journal of cancer
Journal Info 2014 Feb 18;110(4):1066-73
Abstract BACKGROUND: Mutations in HBx gene are frequently found in HBV-associated hepatocellular carcinoma (HCC). Activation of hypoxia-inducible factor-1alpha (HIF-1alpha) contributes to HCC development and progression. Wild-type HBx has been demonstrated to activate HIF-1alpha, but the effect of HBx mutations on HIF-1alpha has not been elucidated. METHODS: HBx mutations were identified by gene sequencing in 101 HCC tissues. Representative HBx mutants were cloned and transfected into HCC cells. Expression and activation of HIF-1alpha were analysed by western blot and luciferase assays, respectively. The relationship between HBx mutants and HIF-1alpha expression in HCC tissues was also evaluated. RESULTS: The dual mutations K130M/V131I enhanced the functionality of HBx as they upregulated the expression and transcriptional activity of HIF-1alpha. The C-terminal truncations and deletion mutations, however, weakened the ability of HBx to upregulate HIF-1alpha. Meanwhile, the C-terminus was further found to be essential for the stability and transactivation of HBx. In the HCC tissues, there was a positive association between the HBx mutants and HIF-1alpha expression. CONCLUSION: Different mutations of HBx exert differentiated effects on the functionality of HIF-1alpha, however, the overall activity of HBx mutants appears to increase the expression and transcriptional activity of HIF-1alpha.
Sequence Data -
Mutation Information
Note
Basic Characteristics of Mutations
  • Mutation Site: The specific location in a gene or protein sequence where a change occurs.
  • Mutation Level: The level at which a mutation occurs, including the nucleotide or amino acid level.
  • Mutation Type: The nature of the mutation, such as missense mutation, nonsense mutation, synonymous mutation, etc.
  • Gene/Protein/Region: Refers to the specific region of the virus where the mutation occurs. Including viral genes, viral proteins, or a specific viral genome region. If the article does not specifically indicate the relationship between the mutation and its correspondence, the main
  • Gene/Protein/Region studied in the article is marked.
  • Genotype/Subtype: Refers to the viral genotype or subtype where the mutation occurs. If the article does not specifically indicate the relationship between the mutation and its correspondence, the main Genotype/Subtype studied in the article is marked.
  • Viral Reference: Refers to the standard virus strain used to compare and analyze viral sequences.
Functional Impact and Mechanisms
  • Disease: An abnormal physiological state with specific symptoms and signs caused by viral infection.
  • Immune: The article focuses on the study of mutations and immune.
  • Target Gene: Host genes that viral mutations may affect.
Clinical and Epidemiological Correlations
  • Clinical Information: The study is a clinical or epidemiological study and provides basic information about the population.
  • Treatment: The study mentioned a certain treatment method, such as drug resistance caused by mutations. If the study does not specifically indicate the relationship between mutations and their correspondence treatment, the main treatment studied in the article is marked.
  • Location: The source of the research data.
Literature Information
  • Sequence Data: The study provides the data accession number.