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Basic Characteristics of Mutations
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Mutation Site
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I123V |
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Mutation Site Sentence
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We found that four amino acid mutations (P224S in the PA protein [PA-P224S], PB2-T588I, NA-V106I, and NS1-I123V) contributed to the lethal phenotype of SD731. |
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Mutation Level
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Amino acid level |
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Mutation Type
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Nonsynonymous substitution |
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Gene/Protein/Region
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NS1 |
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Standardized Encoding Gene
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NS
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Genotype/Subtype
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H1N1 |
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Viral Reference
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-
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Functional Impact and Mechanisms
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Disease
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Influenza A
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Immune
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- |
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Target Gene
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-
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Clinical and Epidemiological Correlations
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Clinical Information
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- |
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Treatment
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- |
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Location
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China;America |
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Literature Information
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PMID
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24522908
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Title
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Naturally occurring mutations in the PA gene are key contributors to increased virulence of pandemic H1N1/09 influenza virus in mice
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Author
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Sun Y,Xu Q,Shen Y,Liu L,Wei K,Sun H,Pu J,Chang KC,Liu J
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Journal
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Journal of virology
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Journal Info
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2014 Apr;88(8):4600-4
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Abstract
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We examined the molecular basis of virulence of pandemic H1N1/09 influenza viruses by reverse genetics based on two H1N1/09 virus isolates (A/California/04/2009 [CA04] and A/swine/Shandong/731/2009 [SD731]) with contrasting pathogenicities in mice. We found that four amino acid mutations (P224S in the PA protein [PA-P224S], PB2-T588I, NA-V106I, and NS1-I123V) contributed to the lethal phenotype of SD731. In particular, the PA-P224S mutation when combined with PA-A70V in CA04 drastically reduced the virus's 50% mouse lethal dose (LD50), by almost 1,000-fold.
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Sequence Data
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HQ533864; HQ533866; HQ533868; HQ533871; HQ533872;HQ533874; HQ533877; HQ533879; JF951848-JF951855;JQ695860-JQ695883;KF918700-KF918707
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