HBV Mutation Detail Information

Virus Mutation HBV Mutation I126S


Basic Characteristics of Mutations
Mutation Site I126S
Mutation Site Sentence Among 40 sequences carrying AA substitution at sI126, sI126T, sI126S, sI126V and sI126N substitution accounted for 70.00% (28/40), 22.50% (9/40), 5.00% (2/40) and 2.50% (1/40), respectively (Fig. 1B).
Mutation Level Amino acid level
Mutation Type Nonsynonymous substitution
Gene/Protein/Region S
Standardized Encoding Gene S  
Genotype/Subtype C2
Viral Reference AF458664;AB042284;AF182804;AY040627;AY050574;M38636
Functional Impact and Mechanisms
Disease Hepatitis B, Chronic    
Immune -
Target Gene -
Clinical and Epidemiological Correlations
Clinical Information Y
Treatment -
Location -
Literature Information
PMID 31442597
Title The modulation of HBsAg level by sI126T is affected by additional amino acid substitutions in the S region of HBV
Author He L,Su M,Ou G,Wang L,Deng J,Zhuang H,Xiang K,Li T
Journal Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases
Journal Info 2019 Nov;75:104006
Abstract The hepatitis B surface antigen (HBsAg) is a vital serum marker for hepatitis B virus (HBV) infection. Amino acid (AA) substitutions in small hepatitis B surface protein (SHBs) are known to affect HBsAg level. However, how the genetic backbones of SHBs sequences would affect the roles of a specific AA substitution on HBsAg level remains unclear. In this study, we found that sI126 had a very high substitution detection rate of 17.54% (40/228) in untreated chronic hepatitis B cohort with subgenotype C2 HBV infection. Among different substitution types at sI126, the sI126T (N = 28) was found to be associated with significantly lower serum HBsAg level. Clone sequencing revealed that sI126T-harboring SHBs sequences had varied genetic backbones with zero to nine additional AA substitutions. Thus, we constructed 24 HBsAg expression plasmids harboring sI126T without (plasmid 1, P1) or with (P2-P24) additional AA substitution(s) and studied them in the HepG2 cells. The HBsAg levels were determined by both ELISA and Western blot. In vitro experiments showed that P1 significantly reduced HBsAg level and its secretion (p < .05), however, P2-P24 showed various extracellular and intracellular HBsAg levels. No significant differences were detected among the HBsAg mRNA levels of nine representative mutant plasmids. Our findings suggest that the modulation of HBsAg level by sI126T is affected by additional AA substitution(s) in the S region of HBV. The effects of AA combination substitutions in SHBs sequences on HBsAg levels are worthwhile for more attentions in terms of HBV biology and its clinical application.
Sequence Data MG714928;MG714949;MG714967;MG714982;MG714991;MG715011;MG715039;MG715054;MG715061;MG715070;MG715082;MG715094;MG715106;MG715118;MG715131;MG715142;MG715150;MG715161;MG715211;MG715226;MG715250;MG715257;MG715268;MG715278;MG715287;MG715295;MG715306;MG715313;MG715320;MG715346;MK343135;MK343142;MK343149;MG714929;MG714951;MG714968;MG714983;MG714994;MG715018;MG715043;MG715055;MG715062;MG715072;MG715086;MG715097;MG715107;MG715120;MG715133;MG715144;MG715152;MG715163;MG715212;MG715228;MG715251;MG715258;MG715269;MG715279;MG715289;MG715296;MG715307;MG715314;MG715321;MG715348;MK343136;MK343143;MG714932;MG714953;MG714971;MG714985;MG714995;MG715025;MG715045;MG715056;MG715063;MG715073;MG715088;MG715099;MG715111;MG715121;MG715136;MG715145;MG715153;MG715164;MG715215;MG715230;MG715252;MG715260;MG715270;MG715280;MG715290;MG715297;MG715308;MG715315;MG715322;MG715349;MK343137;MK343144;MK343150;MG714935;MG714960;MG714978;MG714986;MG714996;MG715032;MG715047;MG715057;MG715064;MG715074;MG715090;MG715101;MG715112;MG715126;MG715137;MG715146;MG715156;MG715203;MG715216;MG715238;MG715253;MG715262;MG715271;MG715281;MG715291;MG715300;MG715309;MG715316;MG715323;MG715351;MK343138;MK343145;MK343151;MG714937;MG714963;MG714979;MG714987;MG714998;MG715035;MG715048;MG715058;MG715066;MG715076;MG715091;MG715103;MG715113;MG715128;MG715138;MG715147;MG715158;MG715207;MG715218;MG715240;MG715254;MG715263;MG715272;MG715282;MG715292;MG715302;MG715310;MG715317;MG715329;MG837059;MK343139;MK343146;MG714938;MG714964;MG714980;MG714988;MG714999;MG715036;MG715049;MG715059;MG715068;MG715078;MG715092;MG715104;MG715115;MG715129;MG715139;MG715148;MG715159;MG715208;MG715221;MG715248;MG715255;MG715264;MG715276;MG715284;MG715293;MG715303;MG715311;MG715318;MG715344;MG837060;MK343140;MK343147;MG714940;MG714965;MG714981;MG714989;MG715005;MG715037;MG715053;MG715060;MG715069;MG715081;MG715093;MG715105;MG715117;MG715130;MG715140;MG715149;MG715160;MG715210;MG715222;MG715249;MG715256;MG715266;MG715277;MG715285;MG715294;MG715305;MG715312;MG715319;MG715345;MG837061;MK343141;MK343148
Mutation Information
Note
Basic Characteristics of Mutations
  • Mutation Site: The specific location in a gene or protein sequence where a change occurs.
  • Mutation Level: The level at which a mutation occurs, including the nucleotide or amino acid level.
  • Mutation Type: The nature of the mutation, such as missense mutation, nonsense mutation, synonymous mutation, etc.
  • Gene/Protein/Region: Refers to the specific region of the virus where the mutation occurs. Including viral genes, viral proteins, or a specific viral genome region. If the article does not specifically indicate the relationship between the mutation and its correspondence, the main
  • Gene/Protein/Region studied in the article is marked.
  • Genotype/Subtype: Refers to the viral genotype or subtype where the mutation occurs. If the article does not specifically indicate the relationship between the mutation and its correspondence, the main Genotype/Subtype studied in the article is marked.
  • Viral Reference: Refers to the standard virus strain used to compare and analyze viral sequences.
Functional Impact and Mechanisms
  • Disease: An abnormal physiological state with specific symptoms and signs caused by viral infection.
  • Immune: The article focuses on the study of mutations and immune.
  • Target Gene: Host genes that viral mutations may affect.
Clinical and Epidemiological Correlations
  • Clinical Information: The study is a clinical or epidemiological study and provides basic information about the population.
  • Treatment: The study mentioned a certain treatment method, such as drug resistance caused by mutations. If the study does not specifically indicate the relationship between mutations and their correspondence treatment, the main treatment studied in the article is marked.
  • Location: The source of the research data.
Literature Information
  • Sequence Data: The study provides the data accession number.