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Basic Characteristics of Mutations
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Mutation Site
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I39V |
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Mutation Site Sentence
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Application of reverse genetics identifies an isoleucine-to-valine mutation (I39V) in the NS2B proteins of both passaged strains that confers enhanced fitness and escape from pre-existing DENV immunity. |
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Mutation Level
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Amino acid level |
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Mutation Type
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Nonsynonymous substitution |
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Gene/Protein/Region
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NS2B |
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Standardized Encoding Gene
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NS2B
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Genotype/Subtype
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- |
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Viral Reference
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KU955593.1
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Functional Impact and Mechanisms
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Disease
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Cell line
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Immune
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Y |
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Target Gene
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-
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Clinical and Epidemiological Correlations
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Clinical Information
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- |
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Treatment
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- |
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Location
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Cambodia |
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Literature Information
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PMID
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35417697
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Title
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A Zika virus mutation enhances transmission potential and confers escape from protective dengue virus immunity
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Author
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Regla-Nava JA,Wang YT,Fontes-Garfias CR,Liu Y,Syed T,Susantono M,Gonzalez A,Viramontes KM,Verma SK,Kim K,Landeras-Bueno S,Huang CT,Prigozhin DM,Gleeson JG,Terskikh AV,Shi PY,Shresta S
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Journal
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Cell reports
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Journal Info
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2022 Apr 12;39(2):110655
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Abstract
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Zika virus (ZIKV) and dengue virus (DENV) are arthropod-borne pathogenic flaviviruses that co-circulate in many countries. To understand some of the pressures that influence ZIKV evolution, we mimic the natural transmission cycle by repeating serial passaging of ZIKV through cultured mosquito cells and either DENV-naive or DENV-immune mice. Compared with wild-type ZIKV, the strains passaged under both conditions exhibit increased pathogenesis in DENV-immune mice. Application of reverse genetics identifies an isoleucine-to-valine mutation (I39V) in the NS2B proteins of both passaged strains that confers enhanced fitness and escape from pre-existing DENV immunity. Introduction of I39V or I39T, a naturally occurring homologous mutation detected in recent ZIKV isolates, increases the replication of wild-type ZIKV in human neuronal precursor cells and laboratory-raised mosquitoes. Our data indicate that ZIKV strains with enhanced transmissibility and pathogenicity can emerge in DENV-naive or -immune settings, and that NS2B-I39 mutants may represent ZIKV variants of interest.
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Sequence Data
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-
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