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Basic Characteristics of Mutations
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Mutation Site
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I440T |
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Mutation Site Sentence
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Amino acid substitution (I440T) may lead to weakened virulence of the epidemic strains. |
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Mutation Level
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Amino acid level |
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Mutation Type
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Nonsynonymous substitution |
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Gene/Protein/Region
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E |
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Standardized Encoding Gene
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Envelope
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Genotype/Subtype
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DENV-1 |
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Viral Reference
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DQ672562
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Functional Impact and Mechanisms
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Disease
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Dengue
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Immune
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- |
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Target Gene
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-
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Clinical and Epidemiological Correlations
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Clinical Information
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Y |
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Treatment
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- |
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Location
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China;Myanmar |
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Literature Information
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PMID
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30528395
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Title
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Molecular characterization of the viral structural gene of the first dengue virus type 1 outbreak in Xishuangbanna: A border area of China, Burma and Laos
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Author
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Lin Y,Ma D,Wen S,Zeng F,Hong S,Li L,Li X,Wang X,Ma Z,Pan Y,Chen J,Xi J,Qiu L,Shan X,Sun Q
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Journal
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International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases
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Journal Info
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2019 Feb;79:152-161
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Abstract
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BACKGROUND: Xishuangbanna, a border area of China, Burma and Laos, had its first major DENV-1 outbreak in 2017. This study aims to explore the genetic characterization, potential source and evolution of the viruses in outbreak. METHODS: The structural protein C/prM/E genes of viruses isolated from local residents or Burmese travelers were sequenced followed by mutation, phylogenetic, homologous recombination, molecular clock and demographic reconstruction analysis. RESULTS: Phylogenetic analysis revealed that all of the strains were classified as three cluster of DENV-1. Cluster 1, 2 and 3 were most similar to China Guangzhou 2011, China Hubei 2014 and Laos 2008 strain, respectively. Among 236 base mutations, 31 caused nonsynonymous mutations when compared with the DENV-1SS. No homologous recombination signal was discovered. The structural protein of these strains had similar three-dimensional structure. Only site 434 showed differences among five predicted protein binding sites. Molecular clock phylogenetic and demographic reconstruction analysis showed that DENV-1 became highly diversified in 1972 followed by a slightly decreased period until 2017. CONCLUSIONS: Dengue isolated strains show diversification between Burma and China. Amino acid substitution (I440T) may lead to weakened virulence of the epidemic strains. DENV-1 became highly diversified in 1972 followed by a slightly decreased period.
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Sequence Data
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MG523256;MG523190;MG696653
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