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Basic Characteristics of Mutations
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Mutation Site
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I84V |
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Mutation Site Sentence
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A human leukocyte antigen (HLA) A0201 restricted epitope, deriving from HIV-1 protease (PR75–84 wt, VLVGPTPVNI) and a mutant variant (PR75–84 d.mut) harboring two drug resistance mutations, V82F and I84V were resuspended in phosphate buffered saline (PBS), biotinylated and coupled to syngeneic erythrocytes by avidin-biotin bridges [5,9-12]. |
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Mutation Level
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Amino acid level |
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Mutation Type
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Nonsynonymous substitution |
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Gene/Protein/Region
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PR |
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Standardized Encoding Gene
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gag-pol
|
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Genotype/Subtype
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HIV-1 |
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Viral Reference
|
-
|
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Functional Impact and Mechanisms
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Disease
|
-
|
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Immune
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- |
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Target Gene
|
-
|
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Clinical and Epidemiological Correlations
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Clinical Information
|
- |
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Treatment
|
PIs |
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Location
|
- |
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Literature Information
|
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PMID
|
17442099
|
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Title
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Immunization with HIV protease peptides linked to syngeneic erythrocytes
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Author
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Boberg A,Dominici S,Brave A,Hallermalm K,Hinkula J,Magnani M,Wahren B
|
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Journal
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Infectious agents and cancer
|
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Journal Info
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2007 Apr 18;2:9
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Abstract
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New potent vaccine adjuvants are desirable for increasing the efficacy of novel vaccine modalities such as DNA and peptides. We therefore tested if syngeneic erythrocytes could serve as delivery vectors for selected HIV peptides and compared the potency of these constructs to immunization with peptides in phosphate buffered saline or in incomplete Freunds adjuvant. Immunization of mice with peptides in a low dose (5 ng) coupled to erythrocytes induced a weak immune response in mice. These peptides alone (5 microg) gave no immune responses, while formulating the peptides (50 microg) in IFA induced strong homologous immunity as well as prominent cross reactivity to a related mutant epitope. Thus, vaccine delivery using syngeneic erythrocytes, although attractive for clinical use, might be of limited value due to the low amount of antigen that can be loaded per erythrocyte.
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Sequence Data
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-
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