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Basic Characteristics of Mutations
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Mutation Site
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K122R |
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Mutation Site Sentence
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Amino acid sequence alignment allowed several known VEMs to be detected, including K122R, K122N, T126A, M133T, and two F134L mutations, in six of the 58 samples tested (Fig. 3a, Table 3 and 4). |
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Mutation Level
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Amino acid level |
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Mutation Type
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Nonsynonymous substitution |
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Gene/Protein/Region
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S |
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Standardized Encoding Gene
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S
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Genotype/Subtype
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B |
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Viral Reference
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-
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Functional Impact and Mechanisms
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Disease
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Hepatitis B Virus Infection
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Immune
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Y |
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Target Gene
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-
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Clinical and Epidemiological Correlations
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Clinical Information
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- |
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Treatment
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- |
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Location
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Japan |
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Literature Information
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PMID
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38632180
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Title
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Characterization of mutations in hepatitis B virus DNA isolated from Japanese HBsAg-positive blood donors in 2021 and 2022
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Author
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Sedohara A,Takahashi K,Arai K,Arizono K,Tuvshinjargal K,Saito M,Nakahara F,Tsutsumi T,Ikeuchi K,Adachi E,Yotsuyanagi H
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Journal
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Archives of virology
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Journal Info
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2024 Apr 18;169(5):103
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Abstract
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Missense mutations in certain small envelope proteins diminish the efficacy of antibodies. Consequently, tracking the incidence and types of vaccine-escape mutations (VEMs) was crucial both before and after the introduction of universal hepatitis B vaccination in Japan in 2016. In this study, we isolated hepatitis B virus (HBV) DNA from 58 of 169 hepatitis B surface antigen (HBsAg)-positive blood samples from Japanese blood donors and determined the nucleotide sequence encoding the small envelope protein. DNA from six (10%) of the samples had VEMs, but no missense mutations, such as G145R, were detected. Complete HBV genome sequences were obtained from 29 of the 58 samples; the viral genotype was A1 in one (3%), A2 in three (10%), B1 in nine (31%), B2 in five (17%), B4 in one (3%), and C2 in 10 (34%) samples. Tenofovir-resistance mutations were detected in two (7%) samples. In addition, several core promoter mutations, such as 1762A>T and 1764G>A, and a precore nonsense mutation, 1986G>A, which are risk factors for HBV-related chronic liver disease, were detected. These findings provide a baseline for future research and highlight the importance of ongoing monitoring of VEMs and drug resistance mutations in HBV DNA from HBsAg-positive blood donors without HBV antibodies.
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Sequence Data
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LC777443;LC777892;LC777893;LC777894;LC777895;LC777896;LC777897;LC777898;LC777899;LC777900;LC777446;LC777901;LC777447;LC777903;LC777905;LC777906;LC777445;LC777907;LC777908;LC777909;LC777910;LC777911;LC777912;LC777913;LC777914;LC777915;LC777916;LC777917;LC777444
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