HIV Mutation Detail Information

Virus Mutation HIV Mutation K158A


Basic Characteristics of Mutations
Mutation Site K158A
Mutation Site Sentence We compared the effect of MI treatment on the single-cycle infectivity of HIV-1 mutants K158A/I and K227A/I and found that none of these IP6 binding-deficient mutant viruses were inhibited by any of the MIs tested, in contrast to WT (Fig. 2, A and B).
Mutation Level Amino acid level
Mutation Type Nonsynonymous substitution
Gene/Protein/Region Gag
Standardized Encoding Gene Gag  
Genotype/Subtype HIV-1
Viral Reference -
Functional Impact and Mechanisms
Disease HIV Infections    
Immune -
Target Gene -
Clinical and Epidemiological Correlations
Clinical Information -
Treatment capsid
Location -
Literature Information
PMID 33692109
Title A stable immature lattice packages IP(6) for HIV capsid maturation
Author Mallery DL,Kleinpeter AB,Renner N,Faysal KMR,Novikova M,Kiss L,Wilson MSC,Ahsan B,Ke Z,Briggs JAG,Saiardi A,Bocking T,Freed EO,James LC
Journal Science advances
Journal Info 2021 Mar 10;7(11):eabe4716
Abstract HIV virion assembly begins with the construction of an immature lattice consisting of Gag hexamers. Upon virion release, protease-mediated Gag cleavage leads to a maturation event in which the immature lattice disassembles and the mature capsid assembles. The cellular metabolite inositiol hexakisphosphate (IP(6)) and maturation inhibitors (MIs) both bind and stabilize immature Gag hexamers, but whereas IP(6) promotes virus maturation, MIs inhibit it. Here we show that HIV is evolutionarily constrained to maintain an immature lattice stability that ensures IP(6) packaging without preventing maturation. Replication-deficient mutant viruses with reduced IP(6) recruitment display increased infectivity upon treatment with the MI PF46396 (PF96) or the acquisition of second-site compensatory mutations. Both PF96 and second-site mutations stabilise the immature lattice and restore IP(6) incorporation, suggesting that immature lattice stability and IP(6) binding are interdependent. This IP(6) dependence suggests that modifying MIs to compete with IP(6) for Gag hexamer binding could substantially improve MI antiviral potency.
Sequence Data -
Mutation Information
Note
Basic Characteristics of Mutations
  • Mutation Site: The specific location in a gene or protein sequence where a change occurs.
  • Mutation Level: The level at which a mutation occurs, including the nucleotide or amino acid level.
  • Mutation Type: The nature of the mutation, such as missense mutation, nonsense mutation, synonymous mutation, etc.
  • Gene/Protein/Region: Refers to the specific region of the virus where the mutation occurs. Including viral genes, viral proteins, or a specific viral genome region. If the article does not specifically indicate the relationship between the mutation and its correspondence, the main
  • Gene/Protein/Region studied in the article is marked.
  • Genotype/Subtype: Refers to the viral genotype or subtype where the mutation occurs. If the article does not specifically indicate the relationship between the mutation and its correspondence, the main Genotype/Subtype studied in the article is marked.
  • Viral Reference: Refers to the standard virus strain used to compare and analyze viral sequences.
Functional Impact and Mechanisms
  • Disease: An abnormal physiological state with specific symptoms and signs caused by viral infection.
  • Immune: The article focuses on the study of mutations and immune.
  • Target Gene: Host genes that viral mutations may affect.
Clinical and Epidemiological Correlations
  • Clinical Information: The study is a clinical or epidemiological study and provides basic information about the population.
  • Treatment: The study mentioned a certain treatment method, such as drug resistance caused by mutations. If the study does not specifically indicate the relationship between mutations and their correspondence treatment, the main treatment studied in the article is marked.
  • Location: The source of the research data.
Literature Information
  • Sequence Data: The study provides the data accession number.