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Basic Characteristics of Mutations
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Mutation Site
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K189R |
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Mutation Site Sentence
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Antigenic cartography demonstrated that the R189K mutation could move A/swine/09SW64/2009(H3N2) from antigenic cluster H3N2SIV-alpha forward to antigenic cluster H3N2SIV-beta whereas the K189R mutation could drive A/swine/11SW347/2011(H3N2) from antigenic cluster H3N2SIV-beta toward antigenic cluster H3N2SIV-alpha (Figure 1). |
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Mutation Level
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Amino acid level |
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Mutation Type
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Nonsynonymous substitution |
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Gene/Protein/Region
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HA |
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Standardized Encoding Gene
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HA
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Genotype/Subtype
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H3N2 |
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Viral Reference
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CY130717.1;CY131957.1
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Functional Impact and Mechanisms
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Disease
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Cell line
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Immune
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- |
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Target Gene
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-
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Clinical and Epidemiological Correlations
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Clinical Information
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- |
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Treatment
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- |
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Location
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America;Australia |
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Literature Information
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PMID
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24074585
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Title
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Mutation from arginine to lysine at the position 189 of hemagglutinin contributes to the antigenic drift in H3N2 swine influenza viruses
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Author
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Ye J,Xu Y,Harris J,Sun H,Bowman AS,Cunningham F,Cardona C,Yoon KJ,Slemons RD,Wan XF
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Journal
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Virology
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Journal Info
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2013 Nov;446(1-2):225-9
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Abstract
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Two distinct antigenic clusters were previously identified among the H3N2 swine influenza A viruses (IAVs) and were designated H3N2SIV-alpha and H3N2SIV-beta (Feng et al., 2013. Journal of Virology 87 (13), 7655-7667). A consistent mutation was observed at the position 189 of hemagglutinin (R189K) between H3N2SIV-alpha and H3N2SIV-beta fair isolates. To evaluate the contribution of R189K mutation to the antigenic drift from H3N2SIV-alpha to H3N2SIV-beta, four reassortant viruses with 189R or 189K were generated. The antigenic cartography demonstrated that the R189K mutation in the hemagglutinin of H3N2 IAV contributed to the antigenic drift, separating these viruses into H3N2SIV-alpha to H3N2SIV-beta. This R189K mutation was also found to contribute to the cross-reaction with several ferret sera raised against historical human IAVs with hemagglutinin carrying 189K. This study suggests that the R189K mutation plays a vital role in the antigenicity of swine and human H3N2 IAVs and identification of this antigenic determinant will help us rapidly identify antigenic variants in influenza surveillance.
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Sequence Data
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-
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