DENV Mutation Detail Information

Virus Mutation DENV Mutation K381E


Basic Characteristics of Mutations
Mutation Site K381E
Mutation Site Sentence Table 2
Mutation Level Amino acid level
Mutation Type Nonsynonymous substitution
Gene/Protein/Region NS3
Standardized Encoding Gene NS3
Genotype/Subtype DENV-3
Viral Reference hDenV3/Sri Lanka/IMTSSA-1266/2000
Functional Impact and Mechanisms
Disease Dengue    
Immune -
Target Gene -
Clinical and Epidemiological Correlations
Clinical Information Y
Treatment -
Location Nepal
Literature Information
PMID 40045383
Title Whole genome sequencing and phylogenetic analysis of dengue virus in Central Nepal from 2022 to 2023
Author Chi M,Katuwal N,Shrestha A,Madhup SK,Tamrakar D,Shrestha R
Journal BMC global and public health
Journal Info 2025 Mar 6;3(1):18
Abstract BACKGROUND: In Nepal, dengue is an emerging disease of growing concern as outbreaks are increasing in both size and geographic reach and beginning to affect areas previously thought dengue-free. Dengue genomic surveillance has previously been limited within Nepal; however, with the increase in accessibility to sequencing technologies since the COVID-19 pandemic, it has recently become more feasible. METHODS: This hospital-based retrospective study utilized banked samples from the 2022 and 2023 dengue seasons from Dhulikhel Hospital/Kathmandu University Hospital in Central Nepal. Next-generation sequencing was performed to obtain whole genome sequences of dengue virus which were analyzed phylogenetically using a maximum likelihood GTR + G model. Mutations were evaluated across viral particle region using the GISAID DengueServer. RESULTS: We obtained 41 full-length sequences of DENV from 80 PCR-positive samples, including 24 sequences (58.5%) from 2022 and 17 sequences (41.5%) from 2023. We identified a shift in the majority serotype of our samples from DENV1 in 2022 to DENV3 in 2023, though 3 out of the 4 serotypes were identified in both years. Phylogenetic analysis revealed clusters within genotype III of DENV1 and genotype III of DENV3 closely related to strains from an outbreak of DENV in northern India in 2018-2019. DENV2 sequences fell into the cosmopolitan genotype IV-A1 and IV-B2 clades and were related to sequences from South and Southeast Asia and the USA, pointing to the global nature of dengue transmission. NS3 showed the highest frequency of mutation, whereas NS2B, NS4, NS5, and E were the most conserved. The most common mutations found were substitutions L17M and T20I in the 2 K peptide. A high number of mutations were observed in DENV3, followed by DENV2, with some mutations being unique to specific serotypes and others matching previously reported strains. CONCLUSIONS: We identified possible clade shifts in the DENV1 and 2 populations and a rising prevalence of DENV3. Our study showed a high level of serotype diversity of DENV circulating in Central Nepal. Furthermore, our results indicate that DENV populations in Nepal are related to a geographically diverse set of sequences but are most strongly influenced by Indian strains of DENV.
Sequence Data EPI_ISL_19081917-EPI_ISL_19081957
Mutation Information
Note
Basic Characteristics of Mutations
  • Mutation Site: The specific location in a gene or protein sequence where a change occurs.
  • Mutation Level: The level at which a mutation occurs, including the nucleotide or amino acid level.
  • Mutation Type: The nature of the mutation, such as missense mutation, nonsense mutation, synonymous mutation, etc.
  • Gene/Protein/Region: Refers to the specific region of the virus where the mutation occurs. Including viral genes, viral proteins, or a specific viral genome region. If the article does not specifically indicate the relationship between the mutation and its correspondence, the main
  • Gene/Protein/Region studied in the article is marked.
  • Genotype/Subtype: Refers to the viral genotype or subtype where the mutation occurs. If the article does not specifically indicate the relationship between the mutation and its correspondence, the main Genotype/Subtype studied in the article is marked.
  • Viral Reference: Refers to the standard virus strain used to compare and analyze viral sequences.
Functional Impact and Mechanisms
  • Disease: An abnormal physiological state with specific symptoms and signs caused by viral infection.
  • Immune: The article focuses on the study of mutations and immune.
  • Target Gene: Host genes that viral mutations may affect.
Clinical and Epidemiological Correlations
  • Clinical Information: The study is a clinical or epidemiological study and provides basic information about the population.
  • Treatment: The study mentioned a certain treatment method, such as drug resistance caused by mutations. If the study does not specifically indicate the relationship between mutations and their correspondence treatment, the main treatment studied in the article is marked.
  • Location: The source of the research data.
Literature Information
  • Sequence Data: The study provides the data accession number.