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Basic Characteristics of Mutations
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Mutation Site
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L217R |
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Mutation Site Sentence
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All cases exhibited the polymorphism rtL217R within the RT domain associated to resistance to adefovir. |
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Mutation Level
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Amino acid level |
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Mutation Type
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Nonsynonymous substitution |
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Gene/Protein/Region
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RT |
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Standardized Encoding Gene
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P
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Genotype/Subtype
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A2;F4;D3;F1b |
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Viral Reference
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-
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Functional Impact and Mechanisms
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Disease
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Occult HBV Infection
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Immune
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- |
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Target Gene
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-
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Clinical and Epidemiological Correlations
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Clinical Information
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Y |
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Treatment
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Abacavir(ADV) |
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Location
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Argentina |
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Literature Information
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PMID
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22445262
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Title
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New natural variants of hepatitis B virus among Amerindians from Argentina with mainly occult infections
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Author
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Delfino CM,Berini C,Eirin ME,Malan R,Pedrozo W,Krupp R,Blejer J,Espejo R,Fierro L,Puca A,Oubina JR,Mathet VL,Biglione MM
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Journal
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Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology
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Journal Info
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2012 Jun;54(2):174-9
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Abstract
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BACKGROUND: Hepatitis B virus infection is frequent among Amerindians. In Argentina HBV genotypes A, B, C, D, E, F and H were described in different populations, while some cases of occult hepatitis B infection (OBI) were reported in human immunodeficiency virus (HIV) coinfected patients. OBJECTIVE: To determine the prevalence, genetic diversity of HBV and to analyze the deduced amino acid sequence of both S and viral polymerase (P) genes among Amerindians of Argentina. STUDY DESIGN: A cross-sectional study including 561 individuals belonging to distinct ethnic groups, the Mbya-guarani (MG), the Kolla (K), the Sagua-Huarpe (SH) and the Wichi (W) was performed. RESULTS: The prevalence of HBsAg was 1.7% and 1.4% for the MG and SH, respectively, while anti-HBc was detected in all communities. HBV DNA of S/P and preCore-Core genomic regions were amplified by nested polymerase chain reaction in 59 reactive samples for anti-HBc total Ig and/or HBsAg. Of them, thirteen exhibited detectable HBV DNA, eleven of which were identified as OBI. Genotype F was predominant in the MG community with co-circulation of subgenotypes F4, F1b, A2 and D3, while subgenotype C2 prevailed within the SH community. All cases exhibited the polymorphism rtL217R within the RT domain associated to resistance to adefovir. Mutations rtD206E and rtV207I associated with lamivudine resistance were found in two MG and three SH respectively. Other new substitutions were described within the P sequence. CONCLUSIONS: This study shows for the first time the predominance of OBI, HBV subgenotypes and natural variants in Amerindians from Argentina.
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Sequence Data
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HQ420153-HQ420168;JN811651-JN811654
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