|
Basic Characteristics of Mutations
|
|
Mutation Site
|
L452L |
|
Mutation Site Sentence
|
For P5-D7, SNP screening and WGS showed the presence only of Omicron (L452L/K471K) while at D1, Delta accounted for 80% of the co-infection. |
|
Mutation Level
|
Amino acid level |
|
Mutation Type
|
Synonymous substitution |
|
Gene/Protein/Region
|
|
|
Standardized Encoding Gene
|
|
|
Genotype/Subtype
|
Delta |
|
Viral Reference
|
-
|
|
Functional Impact and Mechanisms
|
|
Disease
|
-
|
|
Immune
|
- |
|
Target Gene
|
-
|
|
Clinical and Epidemiological Correlations
|
|
Clinical Information
|
- |
|
Treatment
|
- |
|
Location
|
France |
|
Literature Information
|
|
PMID
|
35792280
|
|
Title
|
Evidence of co-infections during Delta and Omicron SARS-CoV-2 variants co-circulation through prospective screening and sequencing
|
|
Author
|
Combes P,Bisseux M,Bal A,Marin P,Latour J,Archimbaud C,Brebion A,Chabrolles H,Regagnon C,Lafolie J,Destras G,Simon B,Izopet J,Laurence Josset,Henquell C,Mirand A
|
|
Journal
|
Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases
|
|
Journal Info
|
2022 Nov;28(11):1503
|
|
Abstract
|
OBJECTIVES: To describe Delta/Omicron SARS-CoV-2 variants co-infection detection and confirmation during the fifth wave of COVID-19 pandemics in France in 7 immunocompetent and epidemiologically unrelated patients. METHODS: Since December 2021, the surveillance of Delta/Omicron SARS-CoV-2 variants of concern (VOC) circulation was performed through prospective screening of positive-samples using single nucleotide polymorphism (SNP) PCR assays targeting SARS-CoV-2 S-gene mutations K417N (Omicron specific) and L452R (Delta specific). Samples showing unexpected mutational profiles were further submitted to whole genome sequencing (WGS) using three different primer sets. RESULTS: Between weeks 49-2021 and 02-2022, SARS-CoV-2 genome was detected in 3831 respiratory samples, of which 3237 (84.5%) were screened for VOC specific SNPs. Unexpected mutation profiles suggesting a dual Delta/Omicron population were observed in 7 nasopharyngeal samples (0.2%). These co-infections were confirmed by WGS. For 2 patients, the sequence analyses of longitudinal samples collected 7 to 11 days apart showed that Delta or Omicron can outcompete the other variant during dual infection. Additionally, for one of these samples, a recombination event between Delta and Omicron was detected. CONCLUSIONS: This work demonstrates that SARS-CoV-2 Delta/Omicron co-infections are not rare in high virus co-circulation periods. Moreover, co-infections can further lead to genetic recombination which may generate new chimeric variants with unpredictable epidemic or pathogenic properties that could represent a serious health threat.
|
|
Sequence Data
|
PRJNA809680
|
|
|