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Basic Characteristics of Mutations
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Mutation Site
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L452R |
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Mutation Site Sentence
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The results presented here suggest that the increased infectivity of BA.5 is not caused by higher viral loads, but presumably by other factors such as increased affinity to human cell receptors or immune escape due to its L452R mutation. |
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Mutation Level
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Amino acid level |
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Mutation Type
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Nonsynonymous substitution |
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Gene/Protein/Region
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Standardized Encoding Gene
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Genotype/Subtype
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Omicron |
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Viral Reference
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-
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Functional Impact and Mechanisms
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Disease
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COVID-19
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Immune
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Y |
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Target Gene
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-
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Clinical and Epidemiological Correlations
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Clinical Information
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Y |
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Treatment
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- |
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Location
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Japan |
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Literature Information
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PMID
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36840495
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Title
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Viral load of SARS-CoV-2 Omicron BA.5 is lower than that of BA.2 despite the higher infectivity of BA.5
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Author
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Takatsuki Y,Takahashi Y,Nakajima J,Iwasaki Y,Nagano K,Tani-Sassa C,Yuasa S,Kanehira S,Sonobe K,Nukui Y,Takeuchi H,Tanimoto K,Tanaka Y,Kimura A,Ichimura N,Tohda S
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Journal
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Immunity, inflammation and disease
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Journal Info
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2023 Feb;11(2):e783
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Abstract
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BACKGROUND: Sublineage BA.5 of the SARS-CoV-2 Omicron variant rapidly spread and replaced BA.2 in July 2022 in Tokyo. A high viral load can be a possible cause of high transmissibility. METHODS AND RESULTS: The copy numbers of SARS-CoV-2 in nasopharyngeal swab samples obtained from all patients visiting the hospital where this research was conducted were measured using reverse transcription-quantitative polymerase chain reaction (RT-qPCR). Viral genotypes were determined using PCR-based melting curve analysis. Next, whole-genome sequencing was performed using approximately one fifth of the samples to verify the viral genotypes determined using PCR. Then, the copy numbers of the BA.1, BA.2, and BA.5 cases were compared. Contrary to expectations, the copy numbers of the BA.5 cases (median 4.7 x 10(4) copies/muL, n = 291) were significantly (p = .001) lower than those of BA.2 cases (median 1.1 x 10(5) copies/muL, n = 184). There was no significant difference (p = .44) between the BA.5 and BA.1 cases (median, 3.3 x 10(4) copies/muL; n = 215). CONCLUSION: The results presented here suggest that the increased infectivity of BA.5 is not caused by higher viral loads, but presumably by other factors such as increased affinity to human cell receptors or immune escape due to its L452R mutation.
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Sequence Data
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-
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