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Basic Characteristics of Mutations
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Mutation Site
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N29S |
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Mutation Site Sentence
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We found that the total genetic variation was as follows: missense mutations D25E(T178G), H78Y(C335T), L83V(T350G), E113D(A442C); and synonymous mutations A131C in E6; and missense mutations A646C (N29H), A647G (N29S), synonymous mutations T846C (S95S) in E7. |
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Mutation Level
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Amino acid level |
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Mutation Type
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Nonsynonymous substitution |
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Gene/Protein/Region
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E7 |
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Standardized Encoding Gene
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E7
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Genotype/Subtype
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HPV16 |
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Viral Reference
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NC_001526
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Functional Impact and Mechanisms
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Disease
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Squamous Intraepithelial Lesions
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Immune
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- |
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Target Gene
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-
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Clinical and Epidemiological Correlations
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Clinical Information
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- |
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Treatment
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- |
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Location
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- |
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Literature Information
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PMID
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27038009
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Title
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Association between human papillomavirus type 16 E6 and E7 variants with subsequent persistent infection and recurrence of cervical high-grade squamous intraepithelial lesion after conization
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Author
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Zhang L,Yang B,Zhang A,Zhou A,Yuan J,Wang Y,Sun L,Cao H,Wang J,Zheng W
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Journal
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Journal of medical virology
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Journal Info
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2016 Nov;88(11):1982-8
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Abstract
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The study aimed to detect the variants of human papillomavirus (HPV) type 16 E6 and E7 in patients with cervical high-grade squamous intraepithelial lesion (HSIL), and to determine the existence and recurrence of persistent infection after treatment with loop electrosurgical excision procedure (LEEP). Preoperatively collected cervical exfoliated cells from 100 HPV 16 positive HSIL patients enrolled in the study were used to test for E6 and E7 variants. Follow-ups which included TCT, HPV test, and colposcopy were performed every 3 months after the operation, and colposcopic biopsy and endocervical curettage were performed for patients with abnormalities. Patients were followed for 2 years, and recurrence was defined as detecting low-grade squamous intraepithelial lesion (LSIL) or relapse of HSIL in 1 year. In 81% of patients, the E6 variant was the Asian prototype (As.P), 14% of patients had the European variant, 2% had the European prototype (EP), and 3% had the African 1 variant (Af1). The HPV16 could be easily cleared by LEEP in patients with As.P. Persistent infection or recurrence was very rare in this group. The patients with European variants T350G or A442C had a significantly higher incidence of persistent and recurring HPV16 infection. In conclusion, (i) in most cases, As.P caused HSIL. (ii) The European variant E6 T350G/A442C may be associated with higher rates of recurring and persistent HPV16 infection after the LEEP. (iii) The E7 gene mutation may not be a risk factor for recurring HSIL caused by HPV16 or persistent infection. J. Med. Virol. 88:1982-1988, 2016. (c) 2016 Wiley Periodicals, Inc.
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Sequence Data
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-
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