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Basic Characteristics of Mutations
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Mutation Site
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N501Y |
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Mutation Site Sentence
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We map the impact of N501Y by structure/function analysis of a large panel of well-characterized monoclonal antibodies. |
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Mutation Level
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Amino acid level |
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Mutation Type
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Nonsynonymous substitution |
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Gene/Protein/Region
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RBD |
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Standardized Encoding Gene
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S
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Genotype/Subtype
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B.1.1.7 |
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Viral Reference
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-
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Functional Impact and Mechanisms
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Disease
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COVID-19
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Immune
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Y |
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Target Gene
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-
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Clinical and Epidemiological Correlations
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Clinical Information
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- |
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Treatment
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- |
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Location
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England |
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Literature Information
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PMID
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33743891
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Title
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Reduced neutralization of SARS-CoV-2 B.1.1.7 variant by convalescent and vaccine sera
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Author
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Supasa P,Zhou D,Dejnirattisai W,Liu C,Mentzer AJ,Ginn HM,Zhao Y,Duyvesteyn HME,Nutalai R,Tuekprakhon A,Wang B,Paesen GC,Slon-Campos J,Lopez-Camacho C,Hallis B,Coombes N,Bewley KR,Charlton S,Walter TS,Barnes E,Dunachie SJ,Skelly D,Lumley SF,Baker N,Shaik I,Humphries HE,Godwin K,Gent N,Sienkiewicz A,Dold C,Levin R,Dong T,Pollard AJ,Knight JC,Klenerman P,Crook D,Lambe T,Clutterbuck E,Bibi S,Flaxman A,Bittaye M,Belij-Rammerstorfer S,Gilbert S,Hall DR,Williams MA,Paterson NG,James W,Carroll MW,Fry EE,Mongkolsapaya J,Ren J,Stuart DI,Screaton GR
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Journal
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Cell
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Journal Info
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2021 Apr 15;184(8):2201-2211
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Abstract
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SARS-CoV-2 has caused over 2 million deaths in little over a year. Vaccines are being deployed at scale, aiming to generate responses against the virus spike. The scale of the pandemic and error-prone virus replication is leading to the appearance of mutant viruses and potentially escape from antibody responses. Variant B.1.1.7, now dominant in the UK, with increased transmission, harbors 9 amino acid changes in the spike, including N501Y in the ACE2 interacting surface. We examine the ability of B.1.1.7 to evade antibody responses elicited by natural SARS-CoV-2 infection or vaccination. We map the impact of N501Y by structure/function analysis of a large panel of well-characterized monoclonal antibodies. B.1.1.7 is harder to neutralize than parental virus, compromising neutralization by some members of a major class of public antibodies through light-chain contacts with residue 501. However, widespread escape from monoclonal antibodies or antibody responses generated by natural infection or vaccination was not observed.
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Sequence Data
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-
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