SARS-CoV-2 Mutation Detail Information

Virus Mutation SARS-CoV-2 Mutation P4715L


Basic Characteristics of Mutations
Mutation Site P4715L
Mutation Site Sentence P4715L and P5828L substitutions in replicase polyprotein were found,as well as D614G in spike protein and L84S in ORF8 in Mexico,Belize,and Guatemala.
Mutation Level Amino acid level
Mutation Type Nonsynonymous substitution
Gene/Protein/Region Replicase
Standardized Encoding Gene ORF1b  
Genotype/Subtype -
Viral Reference NC_045512.2
Functional Impact and Mechanisms
Disease COVID-19    
Immune -
Target Gene -
Clinical and Epidemiological Correlations
Clinical Information -
Treatment -
Location Mexico;Belize;Guatemala
Literature Information
PMID 33049069
Title Analysis of SARS-CoV-2 mutations in Mexico, Belize, and isolated regions of Guatemala and its implication in the diagnosis
Author Hernandez-Huerta MT,Perez-Campos Mayoral L,Romero Diaz C,Martinez Cruz M,Mayoral-Andrade G,Sanchez Navarro LM,Pina-Canseco MDS,Cruz Parada E,Martinez Cruz R,Perez-Campos Mayoral E,Perez Santiago AD,Vasquez Martinez G,Perez-Campos E,Matias-Cervantes CA
Journal Journal of medical virology
Journal Info 2021 Apr;93(4):2099-2114
Abstract The genomic sequences of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) worldwide are publicly available and are derived from studies due to the increase in the number of cases. The importance of study of mutations is related to the possible virulence and diagnosis of SARS-CoV-2. To identify circulating mutations present in SARS-CoV-2 genomic sequences in Mexico, Belize, and Guatemala to find out if the same strain spread to the south, and analyze the specificity of the primers used for diagnosis in these samples. Twenty three complete SARS-CoV-2 genomic sequences, available in the GISAID database from May 8 to September 11, 2020 were analyzed and aligned versus the genomic sequence reported in Wuhan, China (NC_045512.2), using Clustal Omega. Open reading frames were translated using the ExPASy Translate Tool and UCSF Chimera (v.1.12) for amino acid substitutions analysis. Finally, the sequences were aligned versus primers used in the diagnosis of COVID-19. One hundred and eighty seven distinct variants were identified, of which 102 are missense, 66 synonymous and 19 noncoding. P4715L and P5828L substitutions in replicase polyprotein were found, as well as D614G in spike protein and L84S in ORF8 in Mexico, Belize, and Guatemala. The primers design by CDC of United States showed a positive E value. The genomic sequences of SARS-CoV-2 in Mexico, Belize, and Guatemala present similar mutations related to a virulent strain of greater infectivity, which could mean a greater capacity for inclusion in the host genome and be related to an increased spread of the virus in these countries, furthermore, its diagnosis would be affected.
Sequence Data EPI_ISL_426362;EPI_ISL_424667;EPI_ISL_455456;EPI_ISL_426364;EPI_ISL_426363;EPI_ISL_424670;EPI_ISL_424673;EPI_ISL_516613;EPI_ISL_516620;EPI_ISL_454555;EPI_ISL_412972;EPI_ISL_452139;EPI_ISL_424672;EPI_ISL_516625;EPI_ISL_424626;EPI_ISL_455434;EPI_ISL_516609;EPI_ISL_496369;EPI_ISL_493348;EPI_ISL_493336;EPI_ISL_516611;EPI_ISL_455438;EPI_ISL_496374;EPI_ISL_509713;EPI_ISL_509714;EPI_ISL_509712;EPI_ISL_509711;EPI_ISL_509710;EPI_ISL_509700;EPI_ISL_509699;EPI_ISL_509696;EPI_ISL_509695;EPI_ISL_509702;EPI_ISL_509703;EPI_ISL_509697;EPI_ISL_509698;EPI_ISL_509701
Mutation Information
Note
Basic Characteristics of Mutations
  • Mutation Site: The specific location in a gene or protein sequence where a change occurs.
  • Mutation Level: The level at which a mutation occurs, including the nucleotide or amino acid level.
  • Mutation Type: The nature of the mutation, such as missense mutation, nonsense mutation, synonymous mutation, etc.
  • Gene/Protein/Region: Refers to the specific region of the virus where the mutation occurs. Including viral genes, viral proteins, or a specific viral genome region. If the article does not specifically indicate the relationship between the mutation and its correspondence, the main
  • Gene/Protein/Region studied in the article is marked.
  • Genotype/Subtype: Refers to the viral genotype or subtype where the mutation occurs. If the article does not specifically indicate the relationship between the mutation and its correspondence, the main Genotype/Subtype studied in the article is marked.
  • Viral Reference: Refers to the standard virus strain used to compare and analyze viral sequences.
Functional Impact and Mechanisms
  • Disease: An abnormal physiological state with specific symptoms and signs caused by viral infection.
  • Immune: The article focuses on the study of mutations and immune.
  • Target Gene: Host genes that viral mutations may affect.
Clinical and Epidemiological Correlations
  • Clinical Information: The study is a clinical or epidemiological study and provides basic information about the population.
  • Treatment: The study mentioned a certain treatment method, such as drug resistance caused by mutations. If the study does not specifically indicate the relationship between mutations and their correspondence treatment, the main treatment studied in the article is marked.
  • Location: The source of the research data.
Literature Information
  • Sequence Data: The study provides the data accession number.