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Basic Characteristics of Mutations
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Mutation Site
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Q136K |
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Mutation Site Sentence
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Q136K is a novel mutation in NA which confers resistance to zanamivir. |
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Mutation Level
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Amino acid level |
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Mutation Type
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Nonsynonymous substitution |
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Gene/Protein/Region
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NA |
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Standardized Encoding Gene
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NA
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Genotype/Subtype
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H3N2 |
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Viral Reference
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ACI22545.1
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Functional Impact and Mechanisms
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Disease
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-
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Immune
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- |
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Target Gene
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-
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Clinical and Epidemiological Correlations
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Clinical Information
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- |
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Treatment
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zanamivir |
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Location
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- |
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Literature Information
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PMID
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23908028
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Title
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Preliminary crystallographic analysis of neuraminidase N2 from a new influenza A virus
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Author
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Gao F,Bao J
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Journal
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Acta crystallographica. Section F, Structural biology and crystallization communications
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Journal Info
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2013 Aug;69(Pt 8):861-3
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Abstract
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Influenza virus is a major viral respiratory pathogen that causes yearly epidemics in temperate climates. The H3N2 subtype is one of the major causative agents of severe epidemics and plays a critical role in vaccine development. The neuraminidase (NA) inhibitors oseltamivir and zanamivir are two commercially available NA-targeted competitive antiviral drugs. However, their effectiveness has been compromised by the rapid emergence of resistance. Q136K is a novel mutation in NA which confers resistance to zanamivir. In this study, a Q136K mutant N2 protein was expressed in a baculovirus system and crystals were obtained. The crystal of N2 belonged to space group P2(1)2(1)2(1), with unit-cell parameters a = 109.5, b = 112.8, c = 165.2 A. Data were collected to 2.4 A resolution. Four monomers were found in the asymmetric unit. The Matthews coefficient and solvent content were calculated to be 3.0 A(3) Da(-)(1) and 59.0%, respectively.
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Sequence Data
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-
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