HSV1 Mutation Detail Information

Virus Mutation HSV1 Mutation Q27P


Basic Characteristics of Mutations
Mutation Site Q27P
Mutation Site Sentence The two mutants were shown to have different amino acid substitutions (Q27P and Q27R) in gD.
Mutation Level Amino acid level
Mutation Type Nonsynonymous substitution
Gene/Protein/Region gD
Standardized Encoding Gene US6  
Genotype/Subtype -
Viral Reference -
Functional Impact and Mechanisms
Disease Cell line    
Immune -
Target Gene -
Clinical and Epidemiological Correlations
Clinical Information -
Treatment -
Location -
Literature Information
PMID 8116256
Title Single amino acid substitutions in gD of herpes simplex virus 1 confer resistance to gD-mediated interference and cause cell-type-dependent alterations in infectivity
Author Dean HJ,Terhune SS,Shieh MT,Susmarski N,Spear PG
Journal Virology
Journal Info 1994 Feb 15;199(1):67-80
Abstract Previous studies have shown that cell-associated herpes simplex virus (HSV) glycoprotein gD can interfere with infection of the cells by HSV and other alphaherpesviruses and that HSV mutants resistant to this gD-mediated interference can be isolated. Here we report that HSV mutants selected for resistance to gD-mediated interference are altered in specific infectivity for cells that do not express gD. Two independently derived mutants were shown to be impaired in ability to infect HEp-2 cells and enhanced in ability to infect Chinese hamster ovary cells, compared with the wild-type parental strain. The mutants were not significantly different from the parental strain in ability to bind to cells but differed in a postbinding step required for infectivity, probably penetration. The two mutants were shown to have different amino acid substitutions (Q27P and Q27R) in gD. Marker transfer experiments demonstrated that the resistance to gD-mediated interference as well as the altered infectivities resulted from these amino acid substitutions. Thus, small changes in gD structure can not only confer resistance to gD-mediated interference but also alter the relative efficiencies with which HSV penetrates into different cell types.
Sequence Data -
Mutation Information
Note
Basic Characteristics of Mutations
  • Mutation Site: The specific location in a gene or protein sequence where a change occurs.
  • Mutation Level: The level at which a mutation occurs, including the nucleotide or amino acid level.
  • Mutation Type: The nature of the mutation, such as missense mutation, nonsense mutation, synonymous mutation, etc.
  • Gene/Protein/Region: Refers to the specific region of the virus where the mutation occurs. Including viral genes, viral proteins, or a specific viral genome region. If the article does not specifically indicate the relationship between the mutation and its correspondence, the main
  • Gene/Protein/Region studied in the article is marked.
  • Genotype/Subtype: Refers to the viral genotype or subtype where the mutation occurs. If the article does not specifically indicate the relationship between the mutation and its correspondence, the main Genotype/Subtype studied in the article is marked.
  • Viral Reference: Refers to the standard virus strain used to compare and analyze viral sequences.
Functional Impact and Mechanisms
  • Disease: An abnormal physiological state with specific symptoms and signs caused by viral infection.
  • Immune: The article focuses on the study of mutations and immune.
  • Target Gene: Host genes that viral mutations may affect.
Clinical and Epidemiological Correlations
  • Clinical Information: The study is a clinical or epidemiological study and provides basic information about the population.
  • Treatment: The study mentioned a certain treatment method, such as drug resistance caused by mutations. If the study does not specifically indicate the relationship between mutations and their correspondence treatment, the main treatment studied in the article is marked.
  • Location: The source of the research data.
Literature Information
  • Sequence Data: The study provides the data accession number.