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Basic Characteristics of Mutations
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|
Mutation Site
|
Q333R |
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Mutation Site Sentence
|
Strategies employed included exchange of the arginine at position 333 for glutamine and modification of the cytoplasmic domain. |
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Mutation Level
|
Amino acid level |
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Mutation Type
|
Nonsynonymous substitution |
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Gene/Protein/Region
|
G |
|
Standardized Encoding Gene
|
RABVgp4
|
|
Genotype/Subtype
|
- |
|
Viral Reference
|
-
|
|
Functional Impact and Mechanisms
|
|
Disease
|
Rabies Virus infection
|
|
Immune
|
- |
|
Target Gene
|
-
|
|
Clinical and Epidemiological Correlations
|
|
Clinical Information
|
- |
|
Treatment
|
- |
|
Location
|
- |
|
Literature Information
|
|
PMID
|
11348722
|
|
Title
|
Genetic engineering of live rabies vaccines
|
|
Author
|
Morimoto K,McGettigan JP,Foley HD,Hooper DC,Dietzschold B,Schnell MJ
|
|
Journal
|
Vaccine
|
|
Journal Info
|
2001 May 14;19(25-26):3543-51
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Abstract
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Rabies virus is not a single entity but consists of a wide array of variants that are each associated with different host species. These viruses differ greatly in the antigenic makeup of their G proteins, the primary determinant of pathogenicity and major inducer of protective immunity. Due to this diversity, existing rabies vaccines have largely been targeted to individual animal species. In this report, a novel approach to the development of rabies vaccines using genetically modified, reverse-engineered live attenuated rabies viruses is described. This approach entails the engineering of vaccine rabies virus containing G proteins from virulent strains and modification of the G protein to further reduce pathogenicity. Strategies employed included exchange of the arginine at position 333 for glutamine and modification of the cytoplasmic domain. The recombinant viruses obtained were non-neuroinvasive when administered via a peripheral route. The ability to confer protective immunity depended largely upon conservation of the G protein antigenic structure between the vaccine and challenge virus, as well as on the route of immunization.
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Sequence Data
|
-
|