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Basic Characteristics of Mutations
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Mutation Site
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R162K |
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Mutation Site Sentence
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This ts defect is due to a single amino acid replacement (R162K) in a completely conserved region of the NP. |
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Mutation Level
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Amino acid level |
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Mutation Type
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Nonsynonymous substitution |
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Gene/Protein/Region
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NP |
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Standardized Encoding Gene
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NP
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Genotype/Subtype
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- |
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Viral Reference
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-
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Functional Impact and Mechanisms
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Disease
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Cell line
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Immune
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- |
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Target Gene
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-
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Clinical and Epidemiological Correlations
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Clinical Information
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- |
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Treatment
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- |
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Location
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- |
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Literature Information
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PMID
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2014635
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Title
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Mutants and revertants of an avian influenza A virus with temperature-sensitive defects in the nucleoprotein and PB2
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Author
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Mandler J,Muller K,Scholtissek C
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Journal
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Virology
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Journal Info
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1991 Apr;181(2):512-9
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Abstract
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ts19 is a temperature-sensitive (ts) mutant of the influenza A fowl plague virus with a defect in the nucleoprotein (NP). In ts19-infected chicken embryo cells all viral components are synthesized in normal yields at the nonpermissive temperature, but infectious virus is not formed. Under these conditions the migration of the NP and M of ts19 from the cell nucleus to the cytoplasm is affected. This ts defect is due to a single amino acid replacement (R162K) in a completely conserved region of the NP. Another mutant with a different defect in the NP is ts81. After infection with ts81 at 40 degrees no vRNA is being synthesized. By backcross of a revertant derived from ts81 many isolates with a ts defect in the PB2 protein were obtained. This ts defect seems to extragenically suppress the ts defect in the NP gene and to be dominant in a wild-type background.
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Sequence Data
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M24456(FPV); M24660 (ts19); M24556 (19R); M24557 (81R)
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