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Basic Characteristics of Mutations
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Mutation Site
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R5G |
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Mutation Site Sentence
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In the E6 gene, there were four silent mutations and nine missense mutations, resulting in eight amino acid changes (R5G, D32E, D32N, I34L, E36Q, H85Y, L90V, E120D), D32E (54/82, 65.85%) occurred frequently (Table S3). |
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Mutation Level
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Amino acid level |
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Mutation Type
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Nonsynonymous substitution |
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Gene/Protein/Region
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E6 |
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Standardized Encoding Gene
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E6
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Genotype/Subtype
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HPV16 |
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Viral Reference
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NC_001526
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Functional Impact and Mechanisms
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Disease
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Squamous Intraepithelial Lesions
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Immune
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- |
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Target Gene
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-
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Clinical and Epidemiological Correlations
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Clinical Information
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Y |
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Treatment
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- |
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Location
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China |
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Literature Information
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PMID
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31670402
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Title
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Genetic variability and functional implication of HPV16 from cervical intraepithelial neoplasia in Shanghai women
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Author
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Zhao J,Zhu J,Guo J,Zhu T,Zhong J,Liu M,Ruan Y,Liao S,Li F
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Journal
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Journal of medical virology
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Journal Info
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2020 Mar;92(3):372-381
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Abstract
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Human papillomavirus (HPV)16 gene mutation is usually associated with persistent HPV infection and cervical intraepithelial neoplasia (CIN). However, the functional implications of HPV16 mutations remain poorly understood.145 LCR/E6/E7 of the HPV16 isolates were amplified and sequenced, and HPV16 integration status was detected. In total, 89 SNPs (68 in the LCR, 13 in E6, 8 in E7) were discovered, 11 of which were nonsynonymous mutations (8 in E6, 3 in E7). The H85Y and E120D variants in E6 were significantly reduced in the high-grade squamous intraepithelial lesion (HSIL) group compared to the T,"" a potential binding site for TATA-binding protein, is the most common in LCR variants. A4 (Asian) was associated with an increased risk of HSIL compared to A1-3(P = .009). The H85/E120 in E6 and N29 in HPV16 E7 might play a critical role in carcinogenesis by disrupting p53 and Rb degradation due to affecting their interaction, respectively. In a word, the findings in this study provide preventative and therapeutic interventions of HPV16 -related cervical lesions/cancer.
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Sequence Data
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-
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