IV Mutation Detail Information

Virus Mutation IV Mutation R702K


Basic Characteristics of Mutations
Mutation Site R702K
Mutation Site Sentence We introduced 10 mutations found in the clones derived from the N4 replicates (Supplementary Table 2) into the PB2 (PB2-D309N, PB2-R702K), PB1 (PB1-V43I, PB1-V191I, PB1-A661T, PB1-S741F), PA (PA-L54F), and NP (NP-D101N, NP-S287N, NP-M371I) proteins as RNPrib-resist (Fig. 2a).
Mutation Level Amino acid level
Mutation Type Nonsynonymous substitution
Gene/Protein/Region PB2
Standardized Encoding Gene PB2
Genotype/Subtype H3N2;H5N1
Viral Reference A/Wuhan/359/95 wild type;A/Vietnam/1203/04 wild type
Functional Impact and Mechanisms
Disease Cell line    
Immune -
Target Gene -
Clinical and Epidemiological Correlations
Clinical Information -
Treatment -
Location China;Vietnam
Literature Information
PMID 25183443
Title Generation and characterization of influenza A viruses with altered polymerase fidelity
Author Cheung PP,Watson SJ,Choy KT,Fun Sia S,Wong DD,Poon LL,Kellam P,Guan Y,Malik Peiris JS,Yen HL
Journal Nature communications
Journal Info 2014 Sep 3;5:4794
Abstract Genetic diversity of influenza A viruses (IAV) acquired through the error-prone RNA-dependent RNA polymerase (RdRP) or through genetic reassortment enables perpetuation of IAV in humans through epidemics or pandemics. Here, to assess the biological significance of genetic diversity acquired through RdRP, we characterize an IAV fidelity variant derived from passaging a seasonal H3N2 virus in the presence of ribavirin, a purine analogue that increases guanosine-to-adenosine mutations. We demonstrate that a single PB1-V43I mutation increases selectivity to guanosine in A/Wuhan/359/95 (H3N2) and A/Vietnam/1203/04 (H5N1) viruses. The H5N1 PB1-V43I-recombinant virus replicates to comparable titres as the wild-type virus in vitro or in the mouse lungs. However, a decrease in viral population diversity at day 3 post inoculation is associated with a tenfold reduced lethality and neurotropism in mice. Applying a fidelity variant with reduced mutational frequency, we provide direct experimental evidence for the role of genetic diversity in IAV pathogenesis.
Sequence Data -
Mutation Information
Note
Basic Characteristics of Mutations
  • Mutation Site: The specific location in a gene or protein sequence where a change occurs.
  • Mutation Level: The level at which a mutation occurs, including the nucleotide or amino acid level.
  • Mutation Type: The nature of the mutation, such as missense mutation, nonsense mutation, synonymous mutation, etc.
  • Gene/Protein/Region: Refers to the specific region of the virus where the mutation occurs. Including viral genes, viral proteins, or a specific viral genome region. If the article does not specifically indicate the relationship between the mutation and its correspondence, the main
  • Gene/Protein/Region studied in the article is marked.
  • Genotype/Subtype: Refers to the viral genotype or subtype where the mutation occurs. If the article does not specifically indicate the relationship between the mutation and its correspondence, the main Genotype/Subtype studied in the article is marked.
  • Viral Reference: Refers to the standard virus strain used to compare and analyze viral sequences.
Functional Impact and Mechanisms
  • Disease: An abnormal physiological state with specific symptoms and signs caused by viral infection.
  • Immune: The article focuses on the study of mutations and immune.
  • Target Gene: Host genes that viral mutations may affect.
Clinical and Epidemiological Correlations
  • Clinical Information: The study is a clinical or epidemiological study and provides basic information about the population.
  • Treatment: The study mentioned a certain treatment method, such as drug resistance caused by mutations. If the study does not specifically indicate the relationship between mutations and their correspondence treatment, the main treatment studied in the article is marked.
  • Location: The source of the research data.
Literature Information
  • Sequence Data: The study provides the data accession number.