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Basic Characteristics of Mutations
|
|
Mutation Site
|
S122T |
|
Mutation Site Sentence
|
Y56F and S122T provide resistance to the protease inhibitors grazoprevir and simeprevir, respectively. |
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Mutation Level
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Amino acid level |
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Mutation Type
|
Nonsynonymous substitution |
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Gene/Protein/Region
|
NS3 |
|
Standardized Encoding Gene
|
NS3
|
|
Genotype/Subtype
|
1a;1b |
|
Viral Reference
|
-
|
|
Functional Impact and Mechanisms
|
|
Disease
|
Hepatitis C, Chronic
|
|
Immune
|
- |
|
Target Gene
|
-
|
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Clinical and Epidemiological Correlations
|
|
Clinical Information
|
- |
|
Treatment
|
- |
|
Location
|
Brazil |
|
Literature Information
|
|
PMID
|
33259871
|
|
Title
|
Natural prevalence of NS3 gene resistance-associated substitutions (RASs) in patients with chronic hepatitis C from the state of Para/Brazil
|
|
Author
|
da Silva DL,Nunes HM,Freitas PEB
|
|
Journal
|
Virus research
|
|
Journal Info
|
2021 Jan 15;292:198251
|
|
Abstract
|
The resistance of hepatitis C virus (HCV) to direct-acting antiviral agents, used in chronic hepatitis C treatment, consists of a natural process resulting from resistance-associated substitutions (RASs) at specific amino acid regions. To identify and establish the natural prevalence of RASs in the NS3 gene in patients with chronic hepatitis C in the state of Para, northern Brazil. Molecular analysis was performed on a total of 35 patients infected with HCV genotype 1, who were treatment-naive to protease inhibitors. HCV RNA was extracted from plasma and the NS3 region was amplified and submitted to DNA sequencing (Sanger). The general natural prevalence of RASs in the NS3 gene was 37.5 % (Y56F and S122T). The substitutions Y56F (34.3 %), S122T (3.1 %), V132I (15.6 %) and V170I (9.3 %) were identified. Y56F and S122T provide resistance to the protease inhibitors grazoprevir and simeprevir, respectively. All amino acid substitutions in the NS3 gene, including RASs, identified in patients from the state of Para were present in other Brazilian studies. The natural presence of RASs in this study reflects the elevated genetic variability of HCV.
|
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Sequence Data
|
-
|