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Basic Characteristics of Mutations
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Mutation Site
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T1013A |
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Mutation Site Sentence
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By restriction enzyme analysis and sequencing of HDAg-coding region cDNA clones, we found that this HDV isolate bears a novel mutation (T to A) at nucleotide 1013 which converts the amber stop codon (TAG) to a codon for lysine (AAG). |
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Mutation Level
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Nucleotide level |
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Mutation Type
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Gene/Protein/Region
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L-HDAg |
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Standardized Encoding Gene
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LHD
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Genotype/Subtype
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- |
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Viral Reference
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-
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Functional Impact and Mechanisms
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Disease
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Fulminant HDV hepatitis
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Immune
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- |
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Target Gene
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-
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Clinical and Epidemiological Correlations
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Clinical Information
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- |
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Treatment
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- |
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Location
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Central African Republican |
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Literature Information
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PMID
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8376962
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Title
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Discovery of a novel point mutation changing the HDAg expression of a hepatitis delta virus isolate from Central African Republic
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Author
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Tang JR,Hantz O,Vitvitski L,Lamelin JP,Parana R,Cova L,Lesbordes JL,Trepo C
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Journal
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The Journal of general virology
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Journal Info
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1993 Sep;74 ( Pt 9):1827-35
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Abstract
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None of the mutations so far discovered in several hepatitis delta virus (HDV) isolates appears to determine important changes in HDV specific protein (HDAg) expression, except for a putative mutation at nucleotide 1012 converting an amber stop codon (TAG) to a codon for tryptophan (TGG). Here we present the characterization of an HDV obtained from the liver of a woodchuck inoculated with sera from fulminant HDV patients in Central African Republic (CAR). By restriction enzyme analysis and sequencing of HDAg-coding region cDNA clones, we found that this HDV isolate bears a novel mutation (T to A) at nucleotide 1013 which converts the amber stop codon (TAG) to a codon for lysine (AAG). Comparison of these nucleotide sequences with those available from American, Japanese, Taiwanese, French, Italian and Nauru isolates showed a variability of 1.7 to 21.5% and 1.9 to 28.7% at the nucleic acid and amino acid levels, respectively. The HDAg-encoding sequence of the CAR isolate is closely related to that of the Italian HDV isolate. The in vitro expression of this HDV isolate resulted in a unique HDAg species (28K) which was identical with that characterized in vivo.
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Sequence Data
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-
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